Protein Info for EX31_RS13745 in Rahnella sp. WP5

Annotation: pyridoxal kinase PdxY

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00687: pyridoxal kinase
  • amino acids 1 to 285 (285 residues), 421 bits, E=1.2e-130
• PF08543: Phos_pyr_kin
  • amino acids 75 to 263 (189 residues), 52.4 bits, E=5.2e-18
• PF00294: PfkB
  • amino acids 134 to 249 (116 residues), 38.9 bits, E=7e-14

Best Hits

Swiss-Prot: 82% identical to PDXY_YERPS: Pyridoxal kinase PdxY (pdxY) from Yersinia pseudotuberculosis serotype I (strain IP32953)

KEGG orthology group: K00868, pyridoxine kinase [EC: 2.7.1.35] (inferred from 100% identity to rah:Rahaq_2766)

MetaCyc: 78% identical to pyridoxal kinase 2 (Escherichia coli K-12 substr. MG1655)
Pyridoxal kinase. [EC: 2.7.1.35]

Predicted SEED Role

"Pyridoxal kinase (EC 2.7.1.35)" in subsystem Pyridoxin (Vitamin B6) Biosynthesis (EC 2.7.1.35)

MetaCyc Pathways

• superpathway of pyridoxal 5'-phosphate biosynthesis and salvage (12/12 steps found)
• pyridoxal 5'-phosphate salvage I (6/6 steps found)
• pyridoxal 5'-phosphate salvage II (plants) (8/9 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis II (5/8 steps found)

KEGG Metabolic Maps

• Vitamin B6 metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.35

Protein Sequence (286 amino acids)

>EX31_RS13745 pyridoxal kinase PdxY (Rahnella sp. WP5)
MKSILSIQSHTVFGHAGNSAAEFPMRRMGANVWPLNTVQFSNHTQYGHWTGCVMPAAHLT
EIAQGIAEIDRLKDCDAVLSGYIGSPEQGQSILEIVRRVKAANPDAWYFCDPVMGHPEKG
CIVAPGVAEFHCNQALVNCDIIAPNLLELELLSGHAVASVEEAVATARELISKGPKIVLV
KHLSRAGYHSDRFEMLLVTASEAWHIDRPLVDFGVRQPVGVGDLTSGLLLVNLLKGEALD
KALEHVTAAVYEVMLATHAMGEYELQIVAAQDKMVKPEHHFPAVKL