Protein Info for EX31_RS13730 in Rahnella sp. WP5

Annotation: phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 PF00989: PAS" amino acids 9 to 109 (101 residues), 33.5 bits, see alignment E=1.1e-11 TIGR00229: PAS domain S-box protein" amino acids 10 to 123 (114 residues), 47 bits, see alignment E=2.8e-16 PF08448: PAS_4" amino acids 12 to 114 (103 residues), 39.4 bits, see alignment E=1.8e-13 PF13426: PAS_9" amino acids 16 to 116 (101 residues), 38 bits, see alignment E=5e-13 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 128 to 287 (160 residues), 99 bits, see alignment E=2.4e-32 PF00990: GGDEF" amino acids 129 to 285 (157 residues), 126 bits, see alignment E=3.7e-40 PF00563: EAL" amino acids 307 to 537 (231 residues), 212.7 bits, see alignment E=1.6e-66

Best Hits

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_2763)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (557 amino acids)

>EX31_RS13730 phosphodiesterase (Rahnella sp. WP5)
MLTDIAICQAVLDALPEATVLKDNERKLVFLNQKASEFYGRPREELIGQDVSFFTDPEQS
ARQRERDIEVLETGEKTVNEETLVDPSGKRRNVLVRKSRVTLETRHFILTSIMDISLLRS
SEAQIRYLARHDVLTGLPNRTALSEELSQIAARRMYRPDPCALFLLNLNDFNYINDTYGY
QAGDAILCEFGQRLRNEVGNSGTVSRIGGDEFSIILRNNPTSEMANNLALEILVMMRQPF
TLTRANPMITISFGIVMFGAENITAGEIMRRGNTALLEAKQRGKNMFSFYSELLDASSVS
KRIMVKALAESLAKEGDLTCVYQPILRSHDEKVVCVEALARWNHGVLGQVSPVQFIALAE
ETGLVIQLGEIVLRQACRDIRNFERLNLAINVSAVQLGEPDFGPRILAVLAEEDFPPSRL
ELELTETSVMNANGISLSHLAKLRQVGVKISLDDFGTGYSSLSLLKDISVDSVKIDRSFV
QYVTEVNDTAAIVSAVSQLGNKMHLNVIAEGVENQLQKTFLVSAGCSHLQGYLFSRPVPV
EVLSTLLSNEMNMLSST