Protein Info for EX31_RS13715 in Rahnella sp. WP5

Annotation: diguanylate cyclase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 transmembrane" amino acids 576 to 596 (21 residues), see Phobius details PF01590: GAF" amino acids 30 to 152 (123 residues), 49.2 bits, see alignment E=2.9e-16 TIGR00229: PAS domain S-box protein" amino acids 176 to 301 (126 residues), 67.6 bits, see alignment E=1.1e-22 amino acids 342 to 464 (123 residues), 42.1 bits, see alignment E=8.5e-15 PF00989: PAS" amino acids 181 to 291 (111 residues), 30 bits, see alignment E=1.6e-10 PF08448: PAS_4" amino acids 189 to 295 (107 residues), 31.6 bits, see alignment E=5.9e-11 amino acids 352 to 456 (105 residues), 43.6 bits, see alignment E=1.1e-14 PF13426: PAS_9" amino acids 202 to 293 (92 residues), 26.1 bits, see alignment E=3e-09 PF08447: PAS_3" amino acids 203 to 287 (85 residues), 26.6 bits, see alignment E=2.1e-09 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 464 to 629 (166 residues), 139.4 bits, see alignment E=9.4e-45 PF00990: GGDEF" amino acids 468 to 626 (159 residues), 145.8 bits, see alignment E=3.5e-46

Best Hits

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_2760)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (631 amino acids)

>EX31_RS13715 diguanylate cyclase (Rahnella sp. WP5)
MLVPQPAVDEQARLAILASLGILDSPGIEEIDRITRMVARQFQAKAVVVSLIDADRQWFL
SHTGIDIHQSCRDTSFCGHTILEPGPLIVRDARLDLRFHDNPLVTGAPHIVFYAGCALHS
KEGVALGALCLIDDRPRDWCEEEQQTLLDMTALVQSYIFHLENRQYTARVEDNLAKTEAL
FEQTFAHAAVGFSLVGLDWRWLRINPQVCNMLGYAEYQLRACLLHDVTHSDDLYAENVLI
PRLLSGEINSFCIEKLLQRANGSTLWVTLTASLVRNVAGEAHHYIVVISDITERKHIEQA
LYSLQGDLERRVADRTYELQVAMDQLHQEIAQRLTRERELTESKNRLRAITDNIPALICH
VDANEQYTFVNHFHANWYGVEETQVSGKQVRDVVGDDSYQKIKPYIRQALQGMTVSYEIE
LSDHSHHAKGGVLHTTLIPCDDIRNGYYGLSTDISEIKALQAQLEFEANHDALTGLPNRR
AFQQSLIHAAGKQEKADRDIALLFLDIDNFKAYNDTYGHEFGDLILKFFGYTLRENLRSQ
DIVARLAGDEFTVLLSGLMNTESDVSRICISLMSALHSIECIAGVPVALSASIGVAIGRA
RQSLLPDALLARADAAMYRAKQSGKGRYYLG