Protein Info for EX31_RS13620 in Rahnella sp. WP5
Annotation: glycine betaine/L-proline ABC transporter substrate-binding protein ProX
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 69% identical to OUSX_DICD3: Glycine betaine-binding periplasmic protein OusX (ousX) from Dickeya dadantii (strain 3937)
KEGG orthology group: None (inferred from 97% identity to rah:Rahaq_0754)MetaCyc: 68% identical to glycine betaine ABC transporter periplasmic binding protein ProX (Escherichia coli K-12 substr. MG1655)
RXN-8638 [EC: 7.6.2.9]; 7.4.2.- [EC: 7.6.2.9]
Predicted SEED Role
"L-proline glycine betaine binding ABC transporter protein ProX (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)
Isozymes
No predicted isozymesUse Curated BLAST to search for 7.6.2.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (332 amino acids)
>EX31_RS13620 glycine betaine/L-proline ABC transporter substrate-binding protein ProX (Rahnella sp. WP5) MRTTSLNTLVLALSIATPAVFAADLPGKGVVVKPLQSTIAEEAFQTELVNRALEKLGYDV QQTGEVDYNVAYTAIASGDSTYMAVNWEPLQKDMYNTAGGDQKFYRQGTYISGAAQGYLI DKKTADKYHIHDLSQLKDPKIAKLFDADSDGKADMAGCNPGWVCGQVINTQIKAYGLSNT VTQNEGNYSAIIADTLTRFKSGKPVLYFTWTPYWVSNEMKPGKDVVWLTVPFSANPGSQK DLDTTLPNGKNYGFPVNNEHIVANKAWAEKNPAAAKLFAIMKLPLADVNAQNLRMHEGES SSEDIQQHVDGWIKAHQATFDGWIKEAAAAAK