Protein Info for EX31_RS13500 in Rahnella sp. WP5

Annotation: tRNA-modifying protein YgfZ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 330 TIGR03317: folate-binding protein YgfZ" amino acids 188 to 253 (66 residues), 85.7 bits, see alignment E=8e-29 PF21130: YgfZ_barrel" amino acids 245 to 312 (68 residues), 122.2 bits, see alignment E=7.7e-40

Best Hits

Swiss-Prot: 75% identical to YGFZ_SERP5: tRNA-modifying protein YgfZ (Spro_3899) from Serratia proteamaculans (strain 568)

KEGG orthology group: K06980, (no description) (inferred from 100% identity to rah:Rahaq_0775)

Predicted SEED Role

"Folate-dependent protein for Fe/S cluster synthesis/repair in oxidative stress"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (330 amino acids)

>EX31_RS13500 tRNA-modifying protein YgfZ (Rahnella sp. WP5)
MANEFPFPPQKPSASSHLPLTLISLEDWALVTMTGADTVKYLQGQVTADVDALAADQHIL
CAHCDAKGKMWSNLRLFKRGDGMAFIERRNLRDTQLTEIKKYAVFSKVTFTADDDVVLLG
VAGFQASAALTGLFSTLPTAEQQVVQQDETTLLHFSLPAERYLIVTSPEKARQVVEELGE
QAQRNDSQQWLALDIEAGFPVIDTANAVQFIPQATNIHALDGISFTKGCYAGQEMVARAK
YRGANKRALYWLAGKASKVPAPADDLELQLGENWRRTGTVLAAVQLSDGTLWVQAVLNND
LEADASLRVRDDATSQLSIQPLPYSLEETK