Protein Info for EX31_RS13260 in Rahnella sp. WP5

Annotation: glycoside hydrolase family 1 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 41% identical to GMUD_BACSU: 6-phospho-beta-glucosidase GmuD (gmuD) from Bacillus subtilis (strain 168)

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to rah:Rahaq_0821)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

cellulose degradation II (fungi) (2/3 steps found)
neolinustatin bioactivation (2/3 steps found)
linamarin degradation (1/2 steps found)
lotaustralin degradation (1/2 steps found)
linustatin bioactivation (2/4 steps found)
coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
α-tomatine degradation (1/6 steps found)
firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (478 amino acids)

>EX31_RS13260 glycoside hydrolase family 1 protein (Rahnella sp. WP5)
MSKVSIAIPDGFILGAAASAWQTEGWSGKKDGQDSYLDLWYKNNRHVWHNGYGPAKATDF
INRYKEDVALMKASGLSHYRTSVNWSRFFTDYELGTVDEEYAAYIDDLLDEMKRNGIEPM
LCLEHYELPAFLIEKYDGWSSKKVVDLFVLYAEKVFERYAGKVRRWFTFNEPVVVQTRVY
LDAIRWPFEQNTAKWMQWNHHKVLATAKVVELFHRKKYHAAGTVGVILNPEVTYARSSAA
HDQKAAHLYDLFYNRVFLDPMINGEYPQELLELLEKHGVHFDYTEAELAVIRNNTVDEVG
INLYYPHRVKAPSREWNKDTPFHPAYYYEHFELPGRRMNKSRGWEIYPKIIYDMAMRIKA
EYGNISWFVAENGMGIENEGQFRDAVSGEIQDNYRIEFIAEHLYWTLKAREDGANCQGYM
LWAFTDNVSPMNAFKNRYGLIEIDLDNNRDRRQKKSSHWFKSLSESGVLSLTLDDQEK