Protein Info for EX31_RS13230 in Rahnella sp. WP5

Annotation: bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 721 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details transmembrane" amino acids 42 to 63 (22 residues), see Phobius details amino acids 262 to 279 (18 residues), see Phobius details amino acids 418 to 440 (23 residues), see Phobius details PF01553: Acyltransferase" amino acids 30 to 140 (111 residues), 69 bits, see alignment E=5.5e-23 PF00501: AMP-binding" amino acids 223 to 571 (349 residues), 205.1 bits, see alignment E=2.4e-64

Best Hits

Swiss-Prot: 81% identical to AAS_YERPB: Bifunctional protein Aas (aas) from Yersinia pseudotuberculosis serotype IB (strain PB1/+)

KEGG orthology group: K05939, acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase / long-chain-fatty-acid--[acyl-carrier-protein] ligase [EC: 2.3.1.40 6.2.1.20] (inferred from 99% identity to rah:Rahaq_0827)

MetaCyc: 69% identical to fused 2-acylglycerophospho-ethanolamine acyltransferase/acyl-acyl carrier protein synthetase (Escherichia coli K-12 substr. MG1655)
RXN-5741 [EC: 6.2.1.47]; 6.2.1.- [EC: 6.2.1.47]; Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferase. [EC: 6.2.1.47, 2.3.1.40]

Predicted SEED Role

"2-acylglycerophosphoethanolamine acyltransferase (EC 2.3.1.40) / Acyl-[acyl-carrier-protein] synthetase (EC 6.2.1.20)" (EC 2.3.1.40, EC 6.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.40 or 6.2.1.20 or 6.2.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (721 amino acids)

>EX31_RS13230 bifunctional acyl-ACP--phospholipid O-acyltransferase/long-chain-fatty-acid--ACP ligase (Rahnella sp. WP5)
MYNFLFAAFRWLFRLMFRVTVTPARNDFNYPRLILTPNHVSFIDGMLLVLFLPVKPVFAV
YSNITDSWFMRWLKPYVDFMPLDPTKPMAIKHLIREVEKGRPVVVFPEGRITVTGSLMKI
YDGAAFVAAKSQATVIPVRIDGPEYTPFGRLFGSFKIRWFPKVTINLLPPTTIPMPDAPR
SRDRRILAGEQLHKIMMQARMDTREPETLYQALLSAQTRYGRHKPCIADIAFKEDTYQGL
LKKCLGVSRILQRFTREGEHVGMLLPNATIMAAALFGASMRNRIPALLNYTAGAKGLQSA
MKAAQIKTIITSRQFLEKGKLTHLPENVPEANWVYLEDLKDTVTTQDKLWILFHLIFPRR
AMLPQKADDAALVLFTSGSEGNPKGVVHSHASLLANVEQIRTVADFTPRDRFMSSLPLFH
AFGLTVGLLTPLITGARVFLYPSPLHYRIVPELVYDQNCTVLFGTSTFLGNYARFAHPYD
FARLRYVVAGAEKLAESTKEIWQNKFGIRILEGYGVTECAPVGAINVPMATKVNTVGKIL
PGMESRLITVPGIEEGGRLQLRGPNIMKGYLRVENPGVLETPQAENAAGEMQAGWYDTGD
IVTLDEKGFCAIRGRVKRFAKLAGEMVSLESVEQLAIKASPDKQHAATSRSDASKGEALV
LFTTDPELRRDALSKAARELGLPELAVPREIRVVKALPLLGSGKPDFVTLREMAENPESE
A