Protein Info for EX31_RS13200 in Rahnella sp. WP5

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 748 PF13185: GAF_2" amino acids 16 to 154 (139 residues), 55.9 bits, see alignment E=1.7e-18 PF01590: GAF" amino acids 18 to 153 (136 residues), 65.2 bits, see alignment E=2.8e-21 PF13492: GAF_3" amino acids 19 to 152 (134 residues), 40.5 bits, see alignment E=1.1e-13 PF05524: PEP-utilisers_N" amino acids 174 to 294 (121 residues), 90.7 bits, see alignment E=2.5e-29 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 177 to 729 (553 residues), 429.4 bits, see alignment E=9.6e-133 PF00391: PEP-utilizers" amino acids 320 to 391 (72 residues), 59.5 bits, see alignment E=6.3e-20 PF02896: PEP-utilizers_C" amino acids 418 to 707 (290 residues), 365.4 bits, see alignment E=6.3e-113

Best Hits

Swiss-Prot: 78% identical to PT1P_SALTY: Phosphoenolpyruvate-dependent phosphotransferase system (ptsP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K08484, phosphotransferase system, enzyme I, PtsP [EC: 2.7.3.9] (inferred from 78% identity to ecw:EcE24377A_3149)

MetaCyc: 78% identical to phosphoenolpyruvate-protein phosphotransferase PtsP (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase, nitrogen regulation associated"

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.9

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (748 amino acids)

>EX31_RS13200 phosphoenolpyruvate--protein phosphotransferase (Rahnella sp. WP5)
MLTRLREIVEKVAMASSLNDALDVLVNETCLAMDTEVCSVYLADNDRRCYYLMATRGLKK
PRGRTITLAFDQGIVGLVGRLAEPINLADAQSHPSFKYIPAVKEDLFRSFLGVPIIHRRQ
LLGVLVIQQREHRQFDESEESFMVTLATQLAAILSQAQLNVLYGRFRQTRVRALAASPGV
AIGIGWQDSSQPSLDHVYMASTLDTVRERERLTRALEDAGAEFRRFSKRFAASAQKESAA
IFDLYSHLLNDARLKRELFAQIDQGAVAEWAVKKVVEEFAAQFASLQDTYLRERGSDLRA
LGQRLVFHLDDTIQGNTQWPERFILVADELTATLLAEMPQDRLAGVVVRDGAANSHAAIL
VRAMGVPTVMGADIQPALLSQRQLIVDGYRGELLIDPEAVLVTEYTRLISEEMELSQKAE
DDVEQPAALKSGERVKVMLNAGLSADHEKLFGSRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQLFPQKPVTLRTLDIGSDKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
AMLRANAATGNLGILLPMVTSLEEIDEARRLIDRAGREVEEQLGYELPHTRLGVMIEVPS
MVFMLPALAGRVEFISVGTNDLTQYLLAVDRNNTRVAGLYDSLHPAMLRVLKQIVTTAEV
AGLDVSLCGEMAGDPMGALLLTGMGFRNLSMNGRSVARVKYLLRRIELHDAEELAGRVLQ
AHFTTDVRHMTAAFMERRGLGGLIRGGK