Protein Info for EX31_RS12820 in Rahnella sp. WP5

Annotation: DUF3459 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 transmembrane" amino acids 412 to 434 (23 residues), see Phobius details PF00128: Alpha-amylase" amino acids 82 to 345 (264 residues), 69.4 bits, see alignment E=2.2e-23

Best Hits

Swiss-Prot: 49% identical to GGAP_SPITZ: Glucosylglycerate phosphorylase (Spith_0877) from Spirochaeta thermophila (strain ATCC 700085 / DSM 6578 / Z-1203)

KEGG orthology group: K00690, sucrose phosphorylase [EC: 2.4.1.7] (inferred from 100% identity to rah:Rahaq_3885)

Predicted SEED Role

"Putative sucrose phosphorylase (EC 2.4.1.7)" in subsystem Maltose and Maltodextrin Utilization (EC 2.4.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.7

Use Curated BLAST to search for 2.4.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (600 amino acids)

>EX31_RS12820 DUF3459 domain-containing protein (Rahnella sp. WP5)
MSSVNLEYVLRLESLLSRIYPREHIADLLKKILHLADKWNYGKHRTTRWVDGTNIYLITY
GDNIRHGEQPPLATLNHFANTYLRDIISDIHILPMYPYSSDDGFSVIDYRRIDEHLGGWS
DIHHLSANFNLMFDCVINHISRSSEWMKGYLHDDPYYKDYFIAANPELDYSRVVRPRASP
LLSPFIKTDGTELYLWTTFSEDQIDINFKNPQVLLESIDVLLQYAASGGQSIRLDAIGYI
WKEAGTRCIHMPQAHNIIKVWRTILDEVIPGTRIITETNVPHHENISYFGEGDEAHMVYQ
FPLPPLTLHAFLRQDTTTLTQWAQSLNAEARYPDTTYFNFLASHDGIGLRPTETFLTDDE
RKYMAQETLRKEGRVSYKDNGDGTHSPYELNINYLSALTEPEDNTEMKARKFLAAQALLL
SFIGVPAIYIHSLLGSENDLDGMHQSGINRRINRKKLDLMDIEAELEKKDGLRARIFYGL
QALIRHRREHPAFSPQAGQRVFDLGKSLFAMERYDPKTEDRISCIFNISDHSQMLKLAVE
GKDLISGEVFNGQMRLQPWQVVWIKHKLKAPERLGRLRRWRMKLIKRTKRYTISIKRTMR