Protein Info for EX31_RS12280 in Rahnella sp. WP5

Annotation: p-hydroxybenzoic acid efflux pump subunit AaeA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF00529: CusB_dom_1" amino acids 23 to 285 (263 residues), 26.6 bits, see alignment E=9.2e-10 PF16576: HlyD_D23" amino acids 46 to 236 (191 residues), 53.5 bits, see alignment E=4.2e-18 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 241 (196 residues), 118.9 bits, see alignment E=1.1e-38 PF13533: Biotin_lipoyl_2" amino acids 47 to 94 (48 residues), 57.3 bits, see alignment 2e-19 PF13437: HlyD_3" amino acids 157 to 249 (93 residues), 37.6 bits, see alignment E=6.4e-13

Best Hits

Swiss-Prot: 84% identical to AAEA_SERP5: p-hydroxybenzoic acid efflux pump subunit AaeA (aaeA) from Serratia proteamaculans (strain 568)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_4005)

MetaCyc: 72% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (311 amino acids)

>EX31_RS12280 p-hydroxybenzoic acid efflux pump subunit AaeA (Rahnella sp. WP5)
MKNFSIKIIRYTITLILVVLAVIAIFKAWAFYTESPWTRDAKFTADVVSIAPDVTGLITD
VPVTDNQLVKKGQVLFKIDQPRYQQALDEASADVAYYQALASEKKREAGRRVKLGVQAMS
QEEIDQANNVLQTAEHQLAKAQAARDLAVLDLERTVVIAPADGWVTNLTVHPGNYINRGS
TAVALVKKNSFYILAYLEETKLPGLKKGDRAEITPLGSNRIMHGTVDSVAAAVTNSSSSA
ATNGLATIDSNLEWVRLAQRVPVKILLDDKDQQHPYPAGTTATVVITGSHDRKQDEGSPF
LRLLHRLREFG