Protein Info for EX31_RS11975 in Rahnella sp. WP5

Annotation: glycoside hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02922: CBM_48
  • amino acids 51 to 106 (56 residues), 28.5 bits, 1.6e-10
• PF00756: Esterase
  • amino acids 154 to 383 (230 residues), 67.1 bits, E=2e-22

Best Hits

Swiss-Prot: 67% identical to YIEL_ECOLI: Uncharacterized protein YieL (yieL) from Escherichia coli (strain K12)

KEGG orthology group: K07214, enterochelin esterase and related enzymes (inferred from 100% identity to rah:Rahaq_4068)

Predicted SEED Role

"Endo-1,4-beta-xylanase A precursor (EC 3.2.1.8)" in subsystem D-Galacturonate and D-Glucuronate Utilization or Xylose utilization (EC 3.2.1.8)

MetaCyc Pathways

• (1,4)-β-D-xylan degradation (2/2 steps found)
• cellulose and hemicellulose degradation (cellulolosome) (2/3 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.8

Protein Sequence (393 amino acids)

>EX31_RS11975 glycoside hydrolase (Rahnella sp. WP5)
MTRNRLNLATLLILALSAPFPLTVSAQSLPQDPDASVPAKNYITAVNPDLTITYRLYAPT
AEHVDVVTGATPVTYIPHQMTKDDKGVWSWTSGAQKPDLYEYFFNVDGLRIADPGSAMPK
PQRQVNTSLILVPGSILDVKNVPHGEVRTLTYHSAALNSERQVAVWTPPGYTDASQPLPV
LYFYHGFGDTGRSALDQGRIPQIMDNLLAEKKIVPMLVVIPDTETDAKGVVPEDYVTKDR
RKEFYPRNAAAADKELTGDIIPLISKTFRVRDDATGRALAGLSQGGYQTLVSGMTHLDQF
GWLATLSGVSTTTVPNADVAKRFAEPEKINAQLKNFTVVVGEKDVVTGKDIAGLKTELEE
KKIKFDYHEFPNLGHEMDVWRPAYAEFVQKIFK