Protein Info for EX31_RS11835 in Rahnella sp. WP5

Annotation: glycerol-3-phosphate 1-O-acyltransferase PlsB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03703: glycerol-3-phosphate O-acyltransferase
  • amino acids 11 to 799 (789 residues), 1194.3 bits, E=0
• PF01553: Acyltransferase
  • amino acids 282 to 424 (143 residues), 100.5 bits, E=6.5e-33
• PF19277: GPAT_C
  • amino acids 435 to 771 (337 residues), 377.3 bits, E=8.4e-117

Best Hits

Swiss-Prot: 80% identical to PLSB_PECCP: Glycerol-3-phosphate acyltransferase (plsB) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K00631, glycerol-3-phosphate O-acyltransferase [EC: 2.3.1.15] (inferred from 81% identity to ddd:Dda3937_02724)

MetaCyc: 81% identical to glycerol-3-phosphate 1-O-acyltransferase (Escherichia coli K-12 substr. MG1655)
Glycerol-3-phosphate O-acyltransferase. [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]; 2.3.1.15 [EC: 2.3.1.15]

Predicted SEED Role

"Glycerol-3-phosphate acyltransferase (EC 2.3.1.15)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.3.1.15)

MetaCyc Pathways

• superpathway of phospholipid biosynthesis III (E. coli) (11/12 steps found)
• phosphatidylglycerol biosynthesis I (6/6 steps found)
• phosphatidylglycerol biosynthesis II (6/6 steps found)
• CDP-diacylglycerol biosynthesis I (4/4 steps found)
• CDP-diacylglycerol biosynthesis II (4/4 steps found)
• superpathway of cardiolipin biosynthesis (bacteria) (10/13 steps found)
• oleate biosynthesis III (cyanobacteria) (2/3 steps found)
• phosphatidate biosynthesis (yeast) (3/5 steps found)
• diacylglycerol and triacylglycerol biosynthesis (3/7 steps found)
• palmitoyl ethanolamide biosynthesis (2/6 steps found)
• superpathway of stearidonate biosynthesis (cyanobacteria) (2/6 steps found)
• stigma estolide biosynthesis (2/7 steps found)
• anandamide biosynthesis II (2/8 steps found)
• anandamide biosynthesis I (4/12 steps found)
• superpathway of phospholipid biosynthesis II (plants) (10/28 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.15

Use Curated BLAST to search for 2.3.1.15

Protein Sequence (827 amino acids)

>EX31_RS11835 glycerol-3-phosphate 1-O-acyltransferase PlsB (Rahnella sp. WP5)
MSGWRRLYYTLLNLPLSLLVKSKVIPSDPVTELHLDPTRPILYVLPYNSKADLLTLRAQC
LAQNLPDPLIPLEIDGTLLPSHVFIAEGPRVFSYYTPKQESVKLFHDYLDLHRNNPDLDI
QMLPVSVMFGRAPGREGQDDTPHLRVLNGIQKFFAVLWLGRDSFVRFSNTVSLRYMATEH
GTDKTIAQKLARVARMHFSRQRLAAVGPRLPARQDLFNKLLASKAIEKAIEDEARSKKIS
HEKAQQNAIALMEEIAADFSYEAVRLSDRVLSWTWNRLYKGINVNNAERVRQLALDGQEI
VYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNK
LYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLEPKTGTLAMTIQAMLRGGSRPITLV
PIYIGYEHVMEVGTYAKELRGATKEKESLTQMLNGLRKLRNLGQGYVNFGEPLHLTTYLN
QNVPTWREAIDPIEAQRPSWLTPTVQDLAEQIMVRINNSAAANAMNLCCTALLASRQRSL
TREQLTEQIDCYLQLLRNVPYNADSTVPDLTADQLLDHALNMNKFQVEKDNIGDIIVLPR
EQAVLMTYYRNNIHHMLVLPSLISSIVMHHRQVSRAELLHQVELIYPMLKAELFMHFDTP
ELPQVLDVLTLELARQQIIHLEDDELTLNPARIRTLQLLAAGVRETLQRYAITFSLLTNN
PSINRGALEKESRIMAQRLSVLHGINAPEFFDKAVFSTLVGTLRAEGYISDTGDAIQEQT
REIYLMLSDLMTPDIKLTIESAGMPSELPQATEPPAEPKMDVRDTDH