Protein Info for EX31_RS11750 in Rahnella sp. WP5

Annotation: bifunctional isocitrate dehydrogenase kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 570 PF20423: AceK_regulatory" amino acids 8 to 306 (299 residues), 359.3 bits, see alignment E=1.7e-111 PF06315: AceK_kinase" amino acids 309 to 562 (254 residues), 411.9 bits, see alignment E=1.1e-127

Best Hits

Swiss-Prot: 76% identical to ACEK_SERP5: Isocitrate dehydrogenase kinase/phosphatase (aceK) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00906, isocitrate dehydrogenase kinase/phosphatase [EC: 2.7.11.5 3.1.3.-] (inferred from 99% identity to rah:Rahaq_4113)

MetaCyc: 71% identical to isocitrate dehydrogenase kinase/phosphatase (Escherichia coli K-12 substr. MG1655)
3.1.3.-; [Isocitrate dehydrogenase (NADP(+))] kinase. [EC: 2.7.11.5]

Predicted SEED Role

"Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-)" (EC 2.7.11.5, EC 3.1.3.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 2.7.11.5 or 3.1.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (570 amino acids)

>EX31_RS11750 bifunctional isocitrate dehydrogenase kinase/phosphatase (Rahnella sp. WP5)
MPLELLIAQTILQGFDAQYGRFLEVTSGAQQRFENADWSAVQAAMKQRIHLYDHHVGLVV
AQLKCITGQRFYDAAFLIRIKECYSGLLPDYPRAEIAESFFNSVYCRIFKHRDLTADKLF
IFSEQSSRRQQHCARPLSRLYPVQGNFVTTLVKVLDDLPVRLPWEDANRDVDYIATALKQ
RFPAEWLADADIEIATEIFYRNKAAWIIGKIRCASQVFPLLLPVHLSHQNELFVDACLTE
SNDASIVFGFARSYFMVYAPQPGALVEWLREILPAKTTAELYSAIGCQKHAKTECYREYL
HFMAQTDESFIIAPGVKGMVMLVFTLPGSDRVFKVIKDNFAPQKEVTEAQVKACYQLVKE
HDRVGRMADTQEFEHFVLPKNNISPELMAELQREVPAKIEDLGDSIALRHLYIERRMTPL
NLYLEQASDTQLHDVIEEYGNAIKQLAAANIFPGDMLFKNFGVTRHGRVVFYDYDEICYM
TEVNFRDIPAPRYPEDELASEPWYSIAPGDVFPEEFRHFLCSDKRLGQIFETLHDDLFSA
GYWRGLQQRIREGHVEDVFAYRRSRRFSQR