Protein Info for EX31_RS11645 in Rahnella sp. WP5

Annotation: PTS beta-glucoside transporter subunit IIABC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 624 transmembrane" amino acids 107 to 135 (29 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 170 to 190 (21 residues), see Phobius details amino acids 202 to 225 (24 residues), see Phobius details amino acids 244 to 268 (25 residues), see Phobius details amino acids 282 to 308 (27 residues), see Phobius details amino acids 324 to 344 (21 residues), see Phobius details amino acids 356 to 374 (19 residues), see Phobius details amino acids 382 to 404 (23 residues), see Phobius details amino acids 406 to 406 (1 residues), see Phobius details amino acids 427 to 449 (23 residues), see Phobius details TIGR01995: PTS system, beta-glucoside-specific IIABC component" amino acids 3 to 613 (611 residues), 711.6 bits, see alignment E=1.3e-217 PF00367: PTS_EIIB" amino acids 9 to 41 (33 residues), 54.9 bits, see alignment (E = 6.9e-19) PF02378: PTS_EIIC" amino acids 106 to 389 (284 residues), 94.4 bits, see alignment E=1.1e-30 PF00358: PTS_EIIA_1" amino acids 477 to 601 (125 residues), 163.2 bits, see alignment E=3.3e-52 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 477 to 597 (121 residues), 143.7 bits, see alignment E=3.9e-46

Best Hits

Swiss-Prot: 62% identical to PTV3B_DICCH: PTS system beta-glucoside-specific EIIBCA component (arbF) from Dickeya chrysanthemi

KEGG orthology group: K02755, PTS system, beta-glucosides-specific IIA component [EC: 2.7.1.69] K02756, PTS system, beta-glucosides-specific IIB component [EC: 2.7.1.69] K02757, PTS system, beta-glucosides-specific IIC component (inferred from 99% identity to rah:Rahaq_1724)

Predicted SEED Role

"PTS system, beta-glucoside-specific IIB component (EC 2.7.1.69) / PTS system, beta-glucoside-specific IIC component / PTS system, beta-glucoside-specific IIA component" in subsystem Beta-Glucoside Metabolism (EC 2.7.1.69)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (624 amino acids)

>EX31_RS11645 PTS beta-glucoside transporter subunit IIABC (Rahnella sp. WP5)
MEYRTLADEILRGVGGKENVNSVVHCATRLRFKLKDTRKADAAGLKENPGVIMVVESGGQ
FQVVIGNHVGAVYEALVKGSALSDGSDDDRGDNGSVFARAIDVISGIFTPFIGIMAASGI
LKGFLALALACGWMVPESGTYKVWFAASDAMFYFFPLVIGYTAGKKFGGNPFVTMMIGGA
MVHPLMIAAFDVQQSGAHSEHFLGIPLVFINYTSSVIPVILAAWASSWLEKQSMKILPGA
LRNFITPLICLMVIVPLTFLVIGPVATWASQQLAHGYQFVYHVSPMVAGAFMGALWQVCV
IFGLHWGLVPLMINNFSVLGHDTMLPLLLPAVMGQVGAVAGVMLRSKDARTRALSGSAIS
AGIFGITEPAVYGITLPHRRPFIFGCVGGALGAAVLGYFQTTAYSFGFPSVFTFTQIIPP
TGFDKTVTAGIAGTLIALLFAALATYFFGVKQEAVAPEVSEKAQADALQAPRGKTLIASP
MSGPCIALDTVKDPTFASGLLGKGVAIVPVAGRVVSPVDGTVVSLFKTHHAIGIESEDGA
EILIHVGIDTVKLDGKFFTPHVAVDAVVKQGDLLLAFDIQGITEAGYDLTTPVLVTNSDD
YLDVIPACVGQDASEQTLLLTLLR