Protein Info for EX31_RS11465 in Rahnella sp. WP5

Annotation: imidazole glycerol phosphate synthase subunit HisF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00735: imidazoleglycerol phosphate synthase, cyclase subunit
  • amino acids 1 to 256 (256 residues), 385.3 bits, E=5.9e-120
• PF00977: His_biosynth
  • amino acids 5 to 239 (235 residues), 255.3 bits, E=7.4e-80
• PF01207: Dus
  • amino acids 31 to 115 (85 residues), 21.7 bits, E=1.5e-08

Best Hits

Swiss-Prot: 88% identical to HIS6_ECO5E: Imidazole glycerol phosphate synthase subunit HisF (hisF) from Escherichia coli O157:H7 (strain EC4115 / EHEC)

KEGG orthology group: K02500, cyclase [EC: 4.1.3.-] (inferred from 99% identity to rah:Rahaq_1761)

MetaCyc: 88% identical to imidazole glycerol phosphate synthase subunit HisF (Escherichia coli K-12 substr. MG1655)
GLUTAMIDOTRANS-RXN [EC: 4.3.2.10]

Predicted SEED Role

"Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)" in subsystem Histidine Biosynthesis (EC 4.1.3.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• L-histidine biosynthesis (10/10 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-

Use Curated BLAST to search for 4.1.3.- or 4.3.2.10

Protein Sequence (257 amino acids)

>EX31_RS11465 imidazole glycerol phosphate synthase subunit HisF (Rahnella sp. WP5)
MLAKRIIPCLDVKDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGADELVFYDITASSDGRV
VDKSWVSRVAEVIDIPFCVAGGIKSLEDASQILQFGADKISINSPALADPTLITRLADRY
GVQCIVVGIDTWHDTETGTYQVHQFTGDEARTKITTWQTIDWVKEVQERGAGEIVLNMMN
QDGVRNGYDLEQLKMIREVCHVPLIASGGAGTMSHFLDAFREAQVDGALAASVFHKQIIN
IGELKSYLAENGVEIRL