Protein Info for EX31_RS10775 in Rahnella sp. WP5

Annotation: DHA2 family efflux MFS transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 transmembrane" amino acids 22 to 44 (23 residues), see Phobius details amino acids 63 to 82 (20 residues), see Phobius details amino acids 89 to 109 (21 residues), see Phobius details amino acids 115 to 139 (25 residues), see Phobius details amino acids 147 to 170 (24 residues), see Phobius details amino acids 178 to 199 (22 residues), see Phobius details amino acids 212 to 230 (19 residues), see Phobius details amino acids 242 to 259 (18 residues), see Phobius details amino acids 280 to 300 (21 residues), see Phobius details amino acids 316 to 338 (23 residues), see Phobius details amino acids 345 to 368 (24 residues), see Phobius details amino acids 379 to 399 (21 residues), see Phobius details amino acids 410 to 430 (21 residues), see Phobius details amino acids 497 to 515 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 25 to 514 (490 residues), 358.8 bits, see alignment E=2.4e-111 PF07690: MFS_1" amino acids 28 to 422 (395 residues), 123.7 bits, see alignment E=4.3e-40

Best Hits

Swiss-Prot: 40% identical to EMRB_ACIBT: Colistin resistance protein EmrB (emrB) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_1961)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (517 amino acids)

>EX31_RS10775 DHA2 family efflux MFS transporter permease subunit (Rahnella sp. WP5)
MSVTLTPAATLSPADYPTSRKFLIFSIMAFGMFLALIDIQIVAASLNDVQAGLSAGPDEI
SWVQTSYLIAELVMIPFSAFLTQALSTRWLFSASAALFTLSSIGCGLSWNIESMIFFRAL
QGFTGGAMVPTVFATGFAMFQGKQQALIPAILGMVSVLAPTLGPTVGGIVTQLLDWRWIF
FINILPGALVATGAVLFIHVDKADYPMLKRIDWIHLLSMAIALGCLEYVLEEGPRKNWFS
DPQIQISAWFSLVAFVLFLERSFYSKSPIVRLTPFRDPTFTFACLFNLVVGFGLYASTYL
TPVFLGRIRDFNSLQIGTTVFVVGVGQFFSTIIAARLISRVDRRILITVGLSGFALSLWL
TTAVTPYWGAEQFFWPQVVRGLFIMLCIVPSVNMALTAFQGPELRYASGLFNLMRNLGGA
IGIATVNTWLQDWTRVHAARFGESLGQYGDHAQEVMGSLAQKIGHLTPDTAQALLMAKGV
FGAKVAAQSLTLAFNDIFQVMAWMFIAALVMVPFCRK