Protein Info for EX31_RS10670 in Rahnella sp. WP5

Annotation: murein DD-endopeptidase MepM

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 444 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details PF08525: OapA_N" amino acids 14 to 38 (25 residues), 31.6 bits, see alignment (E = 2.9e-11) PF04225: OapA" amino acids 101 to 171 (71 residues), 31 bits, see alignment E=5.6e-11 PF01476: LysM" amino acids 103 to 146 (44 residues), 35.6 bits, see alignment 1.8e-12 PF19425: Csd3_N2" amino acids 184 to 305 (122 residues), 206 bits, see alignment E=3.5e-65 PF01551: Peptidase_M23" amino acids 317 to 411 (95 residues), 122.7 bits, see alignment E=1.4e-39

Best Hits

Swiss-Prot: 74% identical to MEPM_ECOLI: Murein DD-endopeptidase MepM (mepM) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_1990)

MetaCyc: 74% identical to peptidoglycan endopeptidase MepM (Escherichia coli K-12 substr. MG1655)
3.4.-.-; 3.4.-.-

Predicted SEED Role

"Cell wall endopeptidase, family M23/M37"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (444 amino acids)

>EX31_RS10670 murein DD-endopeptidase MepM (Rahnella sp. WP5)
MQLIARSIALAYSNLPRPHRIMLGSLTVVTLAVAVWKPLAYHPDNDLSDTRPLVKNITLD
NQQLRSLLPEASEPIDQSPPADDDIPQDDLDDKTVNEAGVHEYVVSTGDTLSSILNQYGI
DMSEIAALSDANRDLRNLKIGQQISWTLNDAGDLQRLTWEKSRRETYTFDRSGNTFKSSL
ETLKGEWKDSVLKGTLNGSFVTSAKAAGLSSAEVSAVIKALQWQLDFRKLKKGDDFAVLM
SNEVLDGKNEQSELQAVRMRTGGKDYYAVRAEDGKFYDRTGSGLAKGFLRFPTGKQYRVS
SNFNPRRLNPVTGRIAPHRGVDFAVPVGTPVLAVGDGEVVVAKNGGAAGNYVAIRHGRQY
MTRYMHLRKLLVKPGQKVKRGDRIALSGNTGRSTGPHLHFEMWINQLAVNPLTAKLPRTE
GLTGKDRSTYLATVRQVVPQLKLD