Protein Info for EX31_RS10660 in Rahnella sp. WP5

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 transmembrane" amino acids 16 to 33 (18 residues), see Phobius details amino acids 53 to 72 (20 residues), see Phobius details amino acids 84 to 102 (19 residues), see Phobius details amino acids 109 to 130 (22 residues), see Phobius details amino acids 142 to 166 (25 residues), see Phobius details amino acids 172 to 189 (18 residues), see Phobius details amino acids 216 to 239 (24 residues), see Phobius details amino acids 258 to 276 (19 residues), see Phobius details amino acids 287 to 307 (21 residues), see Phobius details amino acids 313 to 335 (23 residues), see Phobius details amino acids 347 to 366 (20 residues), see Phobius details amino acids 371 to 399 (29 residues), see Phobius details PF07690: MFS_1" amino acids 24 to 361 (338 residues), 110.8 bits, see alignment E=7.1e-36 PF00083: Sugar_tr" amino acids 50 to 189 (140 residues), 34.7 bits, see alignment E=1e-12

Best Hits

Swiss-Prot: 59% identical to MDFA_YERPZ: Multidrug transporter MdfA (mdfA) from Yersinia pestis (strain Z176003)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_1992)

MetaCyc: 54% identical to multidrug efflux pump MdfA / Na+:H+ antiporter / K+:H+ antiporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-101; TRANS-RXN-337; TRANS-RXN-338; TRANS-RXN-42; TRANS-RXN-44

Predicted SEED Role

"Multidrug translocase MdfA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (406 amino acids)

>EX31_RS10660 MFS transporter (Rahnella sp. WP5)
MSSVSEPASRLNFRQWLFPLSLVLFEFATYIAHDMIQPGMLQVISDFSASADWVSTSLTA
YLCGGILLQWLLGPLSDRLGRRPVLLTGVAFFTVTCLLTVFTRNIEQFILMRFLQGMSLC
FIGAVGYAAIQEAFSETMSVKLMALMANITLIAPLLGPLAGAAFIHYAPWKAMFALFSLV
SAFSFWGLWRSMPETASGRGERFSMRRLLTDYREVFTNKGVVLGSLSIGLITLPLLAWVA
QSPVLLIQDGGMTPLQYGWWQVPVFTGLIIGNITLSKLSGKVNIRTPVWLGIAPIVLGLA
VSAYAALFMVHAWGWMIGGLSLYALGSGLVNAGLYRLTLFSSPTGKGTVAAALGLLTIVV
FAAGIEVAKQLYFALGNGIFMLAGMISGVTGTLLIAVFLKRVPEKT