Protein Info for EX31_RS10615 in Rahnella sp. WP5

Annotation: ATP-dependent DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 PF00270: DEAD" amino acids 29 to 82 (54 residues), 26.6 bits, see alignment 9.1e-10 PF06733: DEAD_2" amino acids 179 to 247 (69 residues), 22 bits, see alignment E=2.2e-08 PF13307: Helicase_C_2" amino acids 459 to 616 (158 residues), 150 bits, see alignment E=1.4e-47

Best Hits

Swiss-Prot: 80% identical to YOAA_ECOLI: Probable ATP-dependent DNA helicase YoaA (yoaA) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_2001)

MetaCyc: 80% identical to ATP-dependent DNA helicase YoaA (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"DinG family ATP-dependent helicase YoaA" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (637 amino acids)

>EX31_RS10615 ATP-dependent DNA helicase (Rahnella sp. WP5)
MTDDFATDGALAQKIEGFKPREPQRLMAQAVSKAISGKQGLVVEAGTGTGKTYAYLAPAL
RSGKKVIISTGSKNLQDQLYSRDLPKVASALNFKGRLALLKGRSNYLCLERLEQQSLSGG
DLASQALSELAHLRHWSSSTEEGDISTCTVVPEDSFVWPLVTSTNDNCLGSDCPLYKECF
VVKARRKAMDADVVVVNHHLFMADMVVKESGFGELIPEADVMIFDEAHQIPDIASQYFGQ
QVTSRQLLDMAKDITIAYRTEVRDSVQLQKSADRLSQSTMDFRLALGEPGFRGNLRDVLS
QPAIQRALLLLDDALELCYDVVKLSLGRSALLDAAFERATLYRNRLKRLKDVSIPGYSYW
YECNSRHFVLALTPLSVTDKFSEMMKEKPGAWIFTSATLSVNDQLHHFTERLGLDEAETL
LLPSPFDYAKQALLCVPRFLPESNVQGSSKQLARMLRPLIEANKGRCFFLCTSHKMMREL
AEEFRASLTLPVLLQGETSKGQLLAQFVEAGNALLVATSSFWEGVDVRGDALSCVIIDKL
PFTSPDDPLLKARIEDCRLRGGDPFNDVQLPDAVITLKQGVGRLIRDTDDRGVMVICDNR
LVMRPYGEVFLNSLPPTPRTRDLTQAIRFLQNAEEQN