Protein Info for EX31_RS10510 in Rahnella sp. WP5

Annotation: sodium/proton antiporter NhaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 525 transmembrane" amino acids 20 to 63 (44 residues), see Phobius details amino acids 65 to 84 (20 residues), see Phobius details amino acids 92 to 111 (20 residues), see Phobius details amino acids 129 to 162 (34 residues), see Phobius details amino acids 201 to 222 (22 residues), see Phobius details amino acids 242 to 263 (22 residues), see Phobius details amino acids 302 to 337 (36 residues), see Phobius details amino acids 354 to 376 (23 residues), see Phobius details amino acids 379 to 380 (2 residues), see Phobius details amino acids 382 to 382 (1 residues), see Phobius details amino acids 393 to 414 (22 residues), see Phobius details amino acids 455 to 474 (20 residues), see Phobius details amino acids 481 to 500 (20 residues), see Phobius details PF06450: NhaB" amino acids 1 to 513 (513 residues), 927.1 bits, see alignment E=3.3e-283 TIGR00774: Na+/H+ antiporter NhaB" amino acids 1 to 513 (513 residues), 925.7 bits, see alignment E=4.8e-283 PF03600: CitMHS" amino acids 72 to 346 (275 residues), 53.4 bits, see alignment E=2.3e-18

Best Hits

Swiss-Prot: 76% identical to NHAB_SERP5: Na(+)/H(+) antiporter NhaB (nhaB) from Serratia proteamaculans (strain 568)

KEGG orthology group: K03314, Na+:H+ antiporter, NhaB family (inferred from 100% identity to rah:Rahaq_2018)

MetaCyc: 74% identical to Na+:H+ antiporter NhaB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-130

Predicted SEED Role

"Na+/H+ antiporter NhaB" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (525 amino acids)

>EX31_RS10510 sodium/proton antiporter NhaB (Rahnella sp. WP5)
METTYSRAFIKNFLGQSPNWYKIAILVFLIVNPLIFFLISPFIAGWLLVIEFIFTLGMAL
KCYPLQPGGLLAIEAVMIGMTSAGRIREEVSANLEVILLLIFMVAGIYFMKQLLLTVFTK
LLLNLRNKIALSLAFCFAAAFLSAFLDALTVIAVVISVSVGFYSIYHNVASNQNENTEVN
DDSSFTGNQQHQAVLEQFRAFLRSLMMHAGVGTALGGVMTMVGEPQNLIIAKSAGWQFID
FLIRMSPVSVPVFFCGFIVCALVERFRWFGYGAQLPHPVYEVLKKFDQKNSENNTKQERI
KLAVQAVIGVWLIVALAFHLAEVGLIGLTVIIFATALCGVTDEHAIGKAFQDSLPFTALL
TVFFAIVAVIVEQHLFSPIIHFVLQAAPSNQLSLFYLFNGLLSSVSDNVFVGTVYINEAK
SALENGIIDLKQFEMLAVAINTGTNLPSVATPNGQAAFLFLLTSALAPLIRLSYGRMVWM
ALPYTVVMTLVGLLCVQYLLPDMTQWYSDMGWLSLPPVTEIMPVH