Protein Info for EX31_RS10485 in Rahnella sp. WP5

Annotation: D-amino acid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00070: Pyr_redox" amino acids 2 to 36 (35 residues), 24.8 bits, see alignment 1e-08 PF01266: DAO" amino acids 2 to 396 (395 residues), 277.8 bits, see alignment E=7.2e-86 PF02558: ApbA" amino acids 3 to 38 (36 residues), 23.5 bits, see alignment 1.5e-08 PF13450: NAD_binding_8" amino acids 5 to 36 (32 residues), 28.1 bits, see alignment (E = 8e-10)

Best Hits

Swiss-Prot: 82% identical to DADA_SERP5: D-amino acid dehydrogenase (dadA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to rah:Rahaq_2024)

MetaCyc: 79% identical to D-amino acid dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-11193 [EC: 1.4.5.1]; 1.4.5.- [EC: 1.4.5.1]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.5.1 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (433 amino acids)

>EX31_RS10485 D-amino acid dehydrogenase (Rahnella sp. WP5)
MRVVILGSGVVGVASAWYLAKAGHQVTVIDRQSGPAEETSAGNAGQISPGYAAPWAAPGV
PVKAVKWMFQKHAPLAIRLDGTQFQLKWMWQMLRNCNMDHYQTNKSRMVRLAEYSRDCMK
ALREDTGIQYEGRQGGTLQLFRTAQQFESAAKDIAVLEDAGVPYQLLESSQLATAEPALA
QVAHKLTGGLRLPNDETGDCQMFTRKLADMARQAGVIFEFNRTVDKLHIENGEITGVQCG
NEVVRADAYVVAFGSYSTALLRDLVSIPVYPLKGYSLTIPITDPASAPVSTVLDETYKIA
ITRFDDRIRVGGMAEIVGFNLKLAQARRETLEMVVKDLYPNGGNVEQATFWTGLRPMTPD
GTPIVGRTRIKNLYLNTGHGTLGWTMACGSGQLLSDIMSGVTPAIPSDDLSVERYSPDFI
ARPAGPHKMHPVG