Protein Info for EX31_RS10435 in Rahnella sp. WP5

Annotation: biotin-dependent carboxyltransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 333 TIGR00724: biotin-dependent carboxylase uncharacterized domain" amino acids 7 to 322 (316 residues), 292.1 bits, see alignment E=2.3e-91 PF02626: CT_A_B" amino acids 29 to 298 (270 residues), 290.6 bits, see alignment E=6.4e-91

Best Hits

KEGG orthology group: None (inferred from 98% identity to rah:Rahaq_2710)

Predicted SEED Role

"Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54)" (EC 3.5.1.54)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (333 amino acids)

>EX31_RS10435 biotin-dependent carboxyltransferase family protein (Rahnella sp. WP5)
MTEPAVIEIVKTGPLNTVQDAGRNGFRDIGVSVSGVMDPLAFHAGNIMLGNDENAACIEV
QMFPFKVRFLADTSIAITGADCRTKLDGTDLPPWWACSVRAGQELEMQFPRYGARSYLCV
AGGIDVPVVMGSRSTALRGGFGGLDGRPLEKNDRLAAGKTTTSPLPAQGMGIEPPEIALA
NVFPTSTEGCVQLRAIPAGEYSLFEADLPRFWSQPWKVSLHSNRTGYRLSGAPVTPSKVI
EMRSYGLVPGIVQVPPAGEPIIQLADANTAGGYPKIAGIIEEDLWRLAQVQPGQSIQLIK
SDAREAIAVAARISQWLTRLRDSIMPVKKIMGV