Protein Info for EX31_RS09630 in Rahnella sp. WP5

Annotation: spermidine/putrescine ABC transporter substrate-binding protein PotF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 369 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF13531: SBP_bac_11" amino acids 45 to 303 (259 residues), 30.9 bits, see alignment E=6.2e-11 PF01547: SBP_bac_1" amino acids 45 to 302 (258 residues), 70.8 bits, see alignment E=5.2e-23 PF13416: SBP_bac_8" amino acids 46 to 333 (288 residues), 106.1 bits, see alignment E=7.4e-34 PF13343: SBP_bac_6" amino acids 78 to 322 (245 residues), 72.2 bits, see alignment E=1.2e-23

Best Hits

Swiss-Prot: 83% identical to POTF_ECOLI: Putrescine-binding periplasmic protein (potF) from Escherichia coli (strain K12)

KEGG orthology group: K11073, putrescine transport system substrate-binding protein (inferred from 100% identity to rah:Rahaq_2507)

MetaCyc: 83% identical to putrescine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (369 amino acids)

>EX31_RS09630 spermidine/putrescine ABC transporter substrate-binding protein PotF (Rahnella sp. WP5)
MFTLRKKWLSGVVAGLVMAASVTASADEKTLHIYNWSDYIAPNTVPDFQKETGIKVVYDV
FDSNEVLEGKLMAGSTGFDLVVPSASFLERQLAAGVFQPLDKSKLPNYKNMDPELLKMVA
QHDPGNKYAIPYLWATTGIGYNVDKVKAVLGKDAPVDSWDLVLKPENLEKLKSCGVSFLD
APAEIYATVLNYLHLDPNSTKASDYTGAANDLLLKLRPNIRYFHSSQYINDLANGDICVA
IGWAGDVMQAGNRAKEAKNGVNVAYSIPKEGALAFFDVFAIPADAKNLDEAYQFLNYLME
PKVIAQISNTVYYASGNKAATPLVNEDVRNNPGIYPPADVQAKLFTLKVQDPKIDRVITR
SWTKVKTGK