Protein Info for EX31_RS09540 in Rahnella sp. WP5

Annotation: flagellar basal body M-ring protein FliF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 569 transmembrane" amino acids 28 to 48 (21 residues), see Phobius details amino acids 466 to 483 (18 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 18 to 567 (550 residues), 587.4 bits, see alignment E=1.9e-180 PF01514: YscJ_FliF" amino acids 49 to 223 (175 residues), 238.1 bits, see alignment E=5.9e-75 PF08345: YscJ_FliF_C" amino acids 255 to 444 (190 residues), 144.9 bits, see alignment E=2.6e-46

Best Hits

Swiss-Prot: 66% identical to FLIF_ECOLI: Flagellar M-ring protein (fliF) from Escherichia coli (strain K12)

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 100% identity to rah:Rahaq_2489)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (569 amino acids)

>EX31_RS09540 flagellar basal body M-ring protein FliF (Rahnella sp. WP5)
MSSAIAGAETPPSGFTALLNRLRANPKVPIAIAAAAAVAIVVVMMLWAKQPTYKVLFSNL
GDQDGGAIVTQLTQMNIPYQFSDNGGALLIPADKVYETRLKLAAAGLPKGGAVGFELMDQ
EKFGISQFSEQINYQRALEGELSRTIETLGPVSSARVHLAIPKPSLFVREQKNPSASVTL
NLQPGRALDDGQINAIVYMVSSSVSGLPPANVTVVDQSGHLLTQSDNNGRDLNASQLKYA
NEVEGRLQRRIESILQPVVGNGNVHAQITAQIDYASREQTDENYQPNGSADKAAVRSRQL
STSDQIGASSVGGVPGALSNQPAPAATAPVTTPPAANNANGTQNQTGNNAQNAATTANAN
VPSNRRRDETTNYEVDRTITHTQHSAGAVQRLSAAVVVNYQVGPDGKAKPLTDDQLKKID
DLVREAMGFSQERGDTLNVVNTPFNEAADDSGELPFWKQQAFFDQLLNAGRWLLVLIVAW
LLWRKMVRPMLRKQQAEKDAAAAAALAATMPQPEGSKPVKLSNDELEKRKRSQQRVSVEV
QTQRVQELADKDPRIVALVIRQWMSTEQP