Protein Info for EX31_RS09320 in Rahnella sp. WP5

Annotation: nitrate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1254 transmembrane" amino acids 203 to 221 (19 residues), see Phobius details TIGR01580: nitrate reductase, alpha subunit" amino acids 2 to 1237 (1236 residues), 2423.3 bits, see alignment E=0 PF14710: Nitr_red_alph_N" amino acids 3 to 40 (38 residues), 62.2 bits, see alignment (E = 6.6e-21) PF00384: Molybdopterin" amino acids 108 to 834 (727 residues), 238.3 bits, see alignment E=2e-74 PF01568: Molydop_binding" amino acids 1089 to 1202 (114 residues), 66.6 bits, see alignment E=2.9e-22

Best Hits

Swiss-Prot: 84% identical to NARG_ECOLI: Respiratory nitrate reductase 1 alpha chain (narG) from Escherichia coli (strain K12)

KEGG orthology group: K00370, nitrate reductase 1, alpha subunit [EC: 1.7.99.4] (inferred from 75% identity to abo:ABO_0546)

MetaCyc: 84% identical to nitrate reductase A subunit alpha (Escherichia coli K-12 substr. MG1655)
1.97.1.-; RXN0-3501 [EC: 1.7.5.1]; 1.7.5.1 [EC: 1.7.5.1]

Predicted SEED Role

"Respiratory nitrate reductase alpha chain (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.99.4

Use Curated BLAST to search for 1.7.5.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1254 amino acids)

>EX31_RS09320 nitrate reductase subunit alpha (Rahnella sp. WP5)
MSKFLDRFRYFKQLAEPFADGHGQTLNTNRDWEDGYRSRWQHDKVVRSTHGVNCTGSCSW
KIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWYLYSANRLKYPLMRKKLIQ
LWREAKLNYSDPVDAWGSIVNQPEKAKEYKAARGRGGFVRSSWQEVNELIAASNVYTAKT
FGPDRIIGFSPIPAMSMVSYASGARYLSLIGGVCLSFYDWYCDLPPASPQTWGEQTDVPE
SADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGTKTVAITPDYAEIAKICDQWLAPKQ
GTDSALALAMGHVILKSFHIDNPSQYFTDYVRRYTDMPMLVMLEPREDGSYAAGRMLRAS
DLVDNLGQENNPQWKTIAFDENTGELVSPLGSIGYRWGEKGKWNLESREGQDGKDVSLKL
SLLDDHDEVVNVGYPYFGGAVSEHFNSVALDEILMHKLPVKRLQLADGSTGLVASVYDLT
LANYGIERGLNDENCAQGYDEIKAYTPAWAEKITGVSQQNIGRIAHEFADNANKTHGRSM
IIVGAGMNHWYHLDMNYRGLINMLIFCGCVGQSGGGWAHYVGQEKLRPQTGWQPLAFGLD
WQRPPRQMNSTSFFYNHSSQWRYETVAPQELLSPLADKSKFTGSMIDYNVRAERMGWLPS
APQLNVNPLHIAQQAKAAGLSPADFTVAALKDGSVRFAAEQPDNPQNFPRNLFIWRSNLL
GSSGKGHEYMLKYLLGTEHGIQGDDLGIQGGVMPEEVEWQAQGGEGKLDLVVTLDFRMSS
TCLYSDIVLPTATWYEKDDMNTSDMHPFIHPLSAAVDPAWDSKSDWEIYKGIAKEFSRVC
VGHLGVETDMVTLPIQHDSAAELAQPFGVEDWKKGECELIPGRTAPHLMAVERDYPNTYE
RFTSVGPLLEKLGNGGKGISWNTDSEVDLLKKLNYTKADGAAKGQPMINTAIDAAEVILT
LAPETNGQVAVKAWAALSQNTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLED
EHVSYNACYTNVHELIPWRTLSGRQQIYQDHAWMRAFGESLLVYRPPIDTRAAQPLLNAK
PNGNPEMALNFLTPHQKWGIHSTYSDNLLMLTLGRGGPIVWLSEDDATKLGIEDNDWIEA
FNANGSLSARAVVSQRVPAGMTMMYHAQERIVNIPGSEISQQRGGIHNSVTRVCPKPTHM
IGGYAQLAYGFNYYGTVGSNRDEFVIVRKMNRIDWLDDEGNDYVQDAVQQEKAK