Protein Info for EX31_RS09145 in Rahnella sp. WP5

Annotation: 6-phospho-beta-glucosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 PF00232: Glyco_hydro_1" amino acids 4 to 472 (469 residues), 439.1 bits, see alignment E=7.6e-136

Best Hits

Swiss-Prot: 85% identical to ASCB_ECOLI: 6-phospho-beta-glucosidase AscB (ascB) from Escherichia coli (strain K12)

KEGG orthology group: K01223, 6-phospho-beta-glucosidase [EC: 3.2.1.86] (inferred from 99% identity to rah:Rahaq_2398)

MetaCyc: 85% identical to 6-phospho-beta-glucosidase AscB (Escherichia coli K-12 substr. MG1655)
3.2.1.M25 [EC: 3.2.1.M25]; 6-phospho-beta-glucosidase. [EC: 3.2.1.M25, 3.2.1.86]

Predicted SEED Role

"6-phospho-beta-glucosidase ascB (EC 3.2.1.86)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.86)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.86

Use Curated BLAST to search for 3.2.1.86 or 3.2.1.M25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (476 amino acids)

>EX31_RS09145 6-phospho-beta-glucosidase (Rahnella sp. WP5)
MSASAFPEGFLWGGAIAANQAEGGSFEGGKGLTTVDMIPHGAHRLAVKTGQEKRFSLKDD
EFYPAHQAIDFYHRYKEDIALMAEMGFTVFRTSIAWSRLYPNGDELVPNEEGIAFYRDVF
AECKKYGIEPLVTLCHFDVPMHLVIEYGSWRNRKMVEFFARYARTCFEAFDGLVKYWLTF
NEINILLHSPFSGAGLVFEEGENQEQVKYQAAHHELVASALATKIAHEVNPENQVGCMLA
GGNFYPQTCKPADVWAALQKDRENLFFIDVQARGEYPSYTRRVFAEKGVNVVTEPGDAEI
LKNTVDFVSFSYYASRCASADMNDNNSSAANIVKSLKNPYIEASEWGWGIDPLGLRITMN
MMYDRYQKPLFLVENGLGAKDEINAQGEINDDYRISYLREHIRAMGEAITDGVPLIGYTS
WGCIDLVSASTGEMSKRYGFVYVDRDDAGSGTLERKRKKSFWWYKKVIASNGGDLD