Protein Info for EX31_RS08975 in Rahnella sp. WP5

Annotation: protein-disulfide reductase DsbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 180 to 206 (27 residues), see Phobius details amino acids 219 to 243 (25 residues), see Phobius details amino acids 255 to 278 (24 residues), see Phobius details amino acids 300 to 329 (30 residues), see Phobius details amino acids 341 to 360 (20 residues), see Phobius details amino acids 372 to 393 (22 residues), see Phobius details amino acids 399 to 418 (20 residues), see Phobius details amino acids 430 to 447 (18 residues), see Phobius details PF11412: DsbD_N" amino acids 33 to 143 (111 residues), 119.6 bits, see alignment E=2.4e-38 PF13386: DsbD_2" amino acids 182 to 373 (192 residues), 41.9 bits, see alignment E=3.3e-14 PF02683: DsbD_TM" amino acids 182 to 393 (212 residues), 268.1 bits, see alignment E=1.4e-83 PF13899: Thioredoxin_7" amino acids 473 to 550 (78 residues), 47.6 bits, see alignment E=4.4e-16 PF13098: Thioredoxin_2" amino acids 479 to 573 (95 residues), 45.2 bits, see alignment E=3.1e-15 PF00085: Thioredoxin" amino acids 480 to 573 (94 residues), 28.8 bits, see alignment E=3.1e-10

Best Hits

Swiss-Prot: 67% identical to DSBD_YERPP: Thiol:disulfide interchange protein DsbD (dsbD) from Yersinia pestis (strain Pestoides F)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 100% identity to rah:Rahaq_0397)

MetaCyc: 65% identical to thiol-disulfide exchange protein DsbD (Escherichia coli K-12 substr. MG1655)
RXN-19949 [EC: 1.8.4.16]; 1.8.4.16 [EC: 1.8.4.16]; 1.8.4.16 [EC: 1.8.4.16]; RXN0-7311 [EC: 1.8.4.16]

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8 or 1.8.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (578 amino acids)

>EX31_RS08975 protein-disulfide reductase DsbD (Rahnella sp. WP5)
MDHRILRIFFLLCSLSLLPFQANASLFGKPAAPQFVPVDQAFSFDFQQQGHDLKLNWQIR
DGYYLYRSKIELEPKNAQLAAFLMPKGRDHHDEFFGDSEVYENALHLTIPLRQASADATV
RVTYQGCAAAGFCYPPETRDVPLSAVTEPTRVSTPATDTASAPVIVTPEKPSTPLPFSPL
WALLIGIGIAFTPCVLPMYPLISGIILGRQRPHSMKRIFLLALVYVQGMALTYTLLGMVV
AAAGLQFQAALQSPWVLIVLSVMFIVLALSMFGTFSLQLPSAVQTRLALWSNEQKGGSLP
GVFIMGALAGLICSPCTTAPLSAILLYIAQSGNLLAGAGTLYLYALGMGIPLIIVTMFGN
KLLPRSGPWMQYVKEGFGFVILALPVFLLERVIGDIWGLRLWSLLGMAFFGWAFLLSLKS
QRGMVRTLQLLFLAAALIVSRPLQDWAFAPAANTTTQVHALNFQKIHNVGELNAALQGAQ
GKRVMLDLYADWCVACKEFEKYTFSAPDVQNELTDTLLLQADVTQNSPEQKALLERLNVL
GLPTILFFGPDGHELPQSRVTGFMDAAEFSAHLQKTHP