Protein Info for EX31_RS08865 in Rahnella sp. WP5

Annotation: oligoribonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 184 PF00929: RNase_T" amino acids 9 to 170 (162 residues), 129.5 bits, see alignment E=9.1e-42

Best Hits

Swiss-Prot: 88% identical to ORN_YERE8: Oligoribonuclease (orn) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K13288, oligoribonuclease [EC: 3.1.-.-] (inferred from 99% identity to rah:Rahaq_0420)

MetaCyc: 84% identical to oligoribonuclease (Escherichia coli K-12 substr. MG1655)
Oligonucleotidase. [EC: 3.1.13.3]

Predicted SEED Role

"3'-to-5' oligoribonuclease (orn)" in subsystem RNA processing and degradation, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.1.-.-

Use Curated BLAST to search for 3.1.-.- or 3.1.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (184 amino acids)

>EX31_RS08865 oligoribonuclease (Rahnella sp. WP5)
MAANDSNLIWIDLEMTGLDPERDRIIEIATLVTDAHLNILAEGPVMAVHQSDAQLALMDD
WNVRTHTGSGLVDRVKASPFDDNAAQQKTIEFLREWVPAGKSPICGNSVGQDRRFLFKYM
PELEEYFHYRYLDVSTLKELARRWKPEVLAGFKKQNTHQALDDIRESVAELAYYREHFIN
LHNN