Protein Info for EX31_RS08835 in Rahnella sp. WP5

Annotation: N-acetylmuramoyl-L-alanine amidase AmiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details PF11741: AMIN" amino acids 36 to 125 (90 residues), 27.2 bits, see alignment E=5.3e-10 PF01520: Amidase_3" amino acids 202 to 421 (220 residues), 155.2 bits, see alignment E=2.7e-49 PF01476: LysM" amino acids 477 to 519 (43 residues), 49.5 bits, see alignment 4.8e-17 amino acids 544 to 586 (43 residues), 54.9 bits, see alignment 1e-18

Best Hits

KEGG orthology group: K01448, N-acetylmuramoyl-L-alanine amidase [EC: 3.5.1.28] (inferred from 100% identity to rah:Rahaq_0424)

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (588 amino acids)

>EX31_RS08835 N-acetylmuramoyl-L-alanine amidase AmiB (Rahnella sp. WP5)
MNSRFTSLYISAAMLLSTLILFAVLASPVFAAGMKNINVSNGATQSTVTITFDGSPDYSF
FPLHNPERVVMDIRQDGVMKGLPLNFNGQNLVTRVRASTPPNAQSQRLVFELNQKVRTNA
TKQKDGSQYTVVLTMNASAPARQRAPVTVATNTPSPNAGKMITPSEKSNPFTNDQTKVVA
TTVAPDNSRSSTVSTSDSRIIVAIDAGHGGQDPGAIGAGGLKEKNVTISIARKLQAILNA
DPMFQPVLTRDGDYFISVMGRSDVARKKNANVLVSIHADAAPSRSARGASVWVLSNRRAN
SEMGNWLEQHEKQSELLGGAGDVLANTASDPYLSQAVLDLQFGHSQRVGYDVATRVLREL
RSVGSLHKAKPEHASLGVLRSPDIPSLLVETGFISNSTEERLLGSSAYQDKIARAIHIGL
RNYFQSHPIQNGPKVENRPLLASSAAAADSSATTRSAVSQPGPIEVTAGAAGATQIHKVT
RGETLTGIANSYGTTNAALRDLNKLKKDGVWVGQRLKVPAGTRTSSIAAAPDSTAKTAKK
PAKHKVKRGDTLSAIASKYGVSMNDIRTANHMKSGEVQLGQTLTIPQS