Protein Info for EX31_RS08725 in Rahnella sp. WP5

Annotation: bifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01390: 2',3'-cyclic-nucleotide 2'-phosphodiesterase" amino acids 24 to 649 (626 residues), 1144.3 bits, see alignment E=0 PF00149: Metallophos" amino acids 26 to 262 (237 residues), 52 bits, see alignment E=1.3e-17 PF02872: 5_nucleotid_C" amino acids 358 to 552 (195 residues), 75.4 bits, see alignment E=5.2e-25

Best Hits

Swiss-Prot: 80% identical to CPDB_YEREN: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (cpdB) from Yersinia enterocolitica

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] (inferred from 100% identity to rah:Rahaq_0447)

MetaCyc: 78% identical to 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (Escherichia coli K-12 substr. MG1655)
2',3'-cyclic-nucleotide 2'-phosphodiesterase. [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3.1.4.16 [EC: 3.1.4.16]; 3'-nucleotidase. [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]; 3.1.3.6 [EC: 3.1.4.16, 3.1.3.6]

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.6 or 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (649 amino acids)

>EX31_RS08725 bifunctional 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase (Rahnella sp. WP5)
MNKRHLTLSLLAILTAASVQASQVDLRIMETTDLHSNMMDFDYYKDKPTDKFGLVRTASL
IHAARNEVTNSVLVDNGDIIQGSPLGDYMAAKGLKQGDVHPVYLAMNTLDYAVGNLGNHE
FNYGLDYLKKALAGAKFPYVNANVIDDKTGKPLFTPFLITDNAVKDREGKTHNLRIGYIG
FVPPQIMVWDKANLQGKVTVADITETAKKWIPEMRKQGADIIVAIPHSGLSGDPYKTMAE
NSVYYLSQVKGIDAIMFGHAHAVFPSEEFASIKGADIRQGTLNGVPAVMPGMWGDHLGVV
DMVLNNDSGSWKVETAKAEARPIYDKEQKKSLAAEDQSLVKVLAPSHDATRKFVSKPIGK
STDNMYSYLALVQDDPTVQIVNNAQKAYTEHFIQGDPDLADLPVLSAAAPFKVGGRKNDP
ASFVEVEKGELTFRNAADLYLYPNTLVVMKVTGQEVKDWLECSAGQFNQIDIHSTKPQPL
INWDGFRTYNFDVIDGVKYEVDVSQPAKFDGECALINKDAERIKNLTYNGKPIDLKATFL
VATNNYRAYGGKFAGTGDKHIAFASPDENRSVVAAYISAETKKAGAVTPTADNNWKLAKI
AAPQPLDIRFETSPGDKAAAFIQSHAQYPMTAKGTDAIGFEIYQVDLQK