Protein Info for EX31_RS08510 in Rahnella sp. WP5

Annotation: DEAD/DEAH family ATP-dependent RNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 640 PF00270: DEAD" amino acids 31 to 197 (167 residues), 173 bits, see alignment E=1.2e-54 PF04851: ResIII" amino acids 46 to 163 (118 residues), 23.6 bits, see alignment E=1.1e-08 PF00271: Helicase_C" amino acids 233 to 341 (109 residues), 103.2 bits, see alignment E=2.4e-33 PF03880: DbpA" amino acids 496 to 565 (70 residues), 70 bits, see alignment E=3.6e-23 PF12343: DeaD_C" amino acids 585 to 637 (53 residues), 28.5 bits, see alignment 4.3e-10

Best Hits

Swiss-Prot: 83% identical to DEAD_ECOL6: ATP-dependent RNA helicase DeaD (deaD) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K05592, ATP-dependent RNA helicase DeaD [EC: 3.6.4.13] (inferred from 94% identity to rah:Rahaq_0492)

MetaCyc: 83% identical to ATP-dependent RNA helicase DeaD (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (640 amino acids)

>EX31_RS08510 DEAD/DEAH family ATP-dependent RNA helicase (Rahnella sp. WP5)
MTTELETSFADLGLSAPIISALTDLGYEKPSPIQAECIPHLLSGRDVLGMAQTGSGKTAA
FSLPLLHNIDASLKAPQILVLAPTRELAVQVAEAMTDFSKHMHGVNVVALYGGQRYDVQL
RALRQGPQVVVGTPGRLLDHLKRGTLNLSNLSGLVLDEADEMLRMGFIEDVETIMAQIPA
EHQTALFSATMPEAIRRITRRFMKDPQEVRIQSSVTTRPDISQSYWSVYGLRKNEALVRF
LEAEDFDAAIIFVRTKNATLEVAEALERSGYSSAALNGDMNQALREQTLERLKDGRLDIL
IATDVAARGLDVERISLVVNYDIPMDSESYVHRIGRTGRAGRAGRALLFVENRERRLLRN
IERTMKLTIPEVEIPTAEVLGERRLAKFAAKVQQQLESSDLDQYRALLAKLQPAEELDVE
TLAAALLKMAQGERPLIVAADPVFKSRPPRREFEERGDRGDRGDRRDRPVRSNDRPARDG
DSPRRERRDAGEMELYRIEVGRDDGVEVRHIVGAIANEGDISSRYIGNIKLFGTHSTIEL
PKGMPGEILSHFTRTRILNKPMNMQLIGDAVPQAPRRDRPAGAGGERRGNGAPRPFNGER
REGARGTGAPRTFSGDRREGASAAGDRRPPSRAPRRTSDA