Protein Info for EX31_RS08390 in Rahnella sp. WP5

Annotation: alpha,alpha-phosphotrehalase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 547 TIGR02403: alpha,alpha-phosphotrehalase" amino acids 7 to 543 (537 residues), 826.9 bits, see alignment E=4.2e-253 PF00128: Alpha-amylase" amino acids 15 to 476 (462 residues), 571.4 bits, see alignment E=2.8e-175 PF11941: DUF3459" amino acids 459 to 543 (85 residues), 42 bits, see alignment E=1.5e-14 PF16657: Malt_amylase_C" amino acids 476 to 543 (68 residues), 33.1 bits, see alignment E=7.5e-12

Best Hits

Swiss-Prot: 70% identical to TREC_ECOLI: Trehalose-6-phosphate hydrolase (treC) from Escherichia coli (strain K12)

KEGG orthology group: K01226, trehalose-6-phosphate hydrolase [EC: 3.2.1.93] (inferred from 100% identity to rah:Rahaq_0519)

MetaCyc: 70% identical to trehalose-6-phosphate hydrolase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-phosphotrehalase. [EC: 3.2.1.93]

Predicted SEED Role

"Trehalose-6-phosphate hydrolase (EC 3.2.1.93)" in subsystem Trehalose Uptake and Utilization (EC 3.2.1.93)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.93

Use Curated BLAST to search for 3.2.1.93

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (547 amino acids)

>EX31_RS08390 alpha,alpha-phosphotrehalase (Rahnella sp. WP5)
MNSELPWWKNGVIYQIYPKSFQDTTGSGTGDINGITQRLDYLKTLGVDAIWLTPMYLSPQ
IDNGYDVADYCEIDPAYGTLEDFERLTAEAHLRGIRIVMDMVFNHTSTHHEWFRQSQDAG
SPFRPFYIWRDGATEGGPPNNWRSKFGGGAWQWHETSGQYYLHLFATEQADLNWEYPPVR
EALKKVCQFWADKGVDGLRLDVINLVSKQQDFPTDDAGDGRRFYTDGPRIHEFLQEFSRD
VFKPLGLMTVGEMSSTKLEHCRRYAANDGSELSMTFNFHHLKVDYPNGEKWADAPPDFVQ
LKQIFREWQQGMHGHAWNALFWCNHDQPRIVSRFGDEGALRTTSAKMLAMVLHGLQGTPY
IYQGEEIGMTNPGFEHLEQYRDVESLNMFAELKTDGRSNEELLRILARKSRDNSRTPMQW
NAGEQAGFTSGTPWISVSHNAQDINTEQALADKDSVFYLYQQLIRLRKTLPVLTDGDYQD
LLPEHPQLWCYTRQASGKTLRVVANLSGQEQTLTLAELDGNTLLSNYASPAISGTLRPYE
CLWQLAG