Protein Info for EX31_RS08375 in Rahnella sp. WP5

Annotation: magnesium-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 signal peptide" amino acids 1 to 49 (49 residues), see Phobius details transmembrane" amino acids 124 to 143 (20 residues), see Phobius details amino acids 245 to 263 (19 residues), see Phobius details amino acids 294 to 315 (22 residues), see Phobius details amino acids 323 to 347 (25 residues), see Phobius details amino acids 709 to 734 (26 residues), see Phobius details amino acids 770 to 793 (24 residues), see Phobius details amino acids 805 to 823 (19 residues), see Phobius details amino acids 835 to 857 (23 residues), see Phobius details amino acids 871 to 893 (23 residues), see Phobius details TIGR01524: magnesium-translocating P-type ATPase" amino acids 44 to 900 (857 residues), 1426.1 bits, see alignment E=0 PF00690: Cation_ATPase_N" amino acids 56 to 118 (63 residues), 41.9 bits, see alignment 1.9e-14 TIGR01494: HAD ATPase, P-type, family IC" amino acids 130 to 397 (268 residues), 117.2 bits, see alignment E=6.9e-38 amino acids 608 to 730 (123 residues), 104.1 bits, see alignment E=6.3e-34 PF00122: E1-E2_ATPase" amino acids 158 to 357 (200 residues), 136.4 bits, see alignment E=2.5e-43 PF00702: Hydrolase" amino acids 374 to 655 (282 residues), 56.7 bits, see alignment E=1.3e-18 PF13246: Cation_ATPase" amino acids 438 to 481 (44 residues), 36.3 bits, see alignment 1.4e-12 PF00689: Cation_ATPase_C" amino acids 724 to 890 (167 residues), 75.6 bits, see alignment E=1.3e-24

Best Hits

Swiss-Prot: 73% identical to ATMA_ECO57: Magnesium-transporting ATPase, P-type 1 (mgtA) from Escherichia coli O157:H7

KEGG orthology group: K01531, Mg2+-importing ATPase [EC: 3.6.3.2] (inferred from 73% identity to ecl:EcolC_3769)

Predicted SEED Role

"Magnesium transporting ATPase, P-type 1 (EC 3.6.3.2)" (EC 3.6.3.2)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.2

Use Curated BLAST to search for 3.6.3.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (900 amino acids)

>EX31_RS08375 magnesium-translocating P-type ATPase (Rahnella sp. WP5)
MMFKNFTSRLLSALSRNLPRRLVRRDPMLENVSARAVQAIPAAMSAHCQACARATEDQLY
LQFSSHPEGLTAQEADAIREVSGLNQTGDQQPAPWWLHLWHCYRNPFNLLLTVLGLVSYA
TDDLTAALVIGAMVFISTFMHFIQEARSNKAADALKAMVSNTATVLRSDAQTGRSETVEI
PISQLVPGDLIKLSAGDMIPADLRILSAKDLFISQAALTGESLPVEKHACEKKPVAADPL
ELNTLCFMGTNVVSGTAIAMVIATGSKTWFGQLAERVVQEDTQPNAFQAGISKVSWLLIR
FMMVMTPVVLVINGYTKGDWWEAALFALSVAVGLTPEMLPMIVTSTLAKGAVKLSRQKVI
VKRLDAIQNFGAMDILCTDKTGTLTQDKIVLERHTDVLGVTSDEVLHLAWLNSHYQTGLK
NLLDVAVLEAGDAGPAHNSLKVDEIPFDFDRRRMSVVVAENDQNHRLICKGALEEMLSIC
TLVQLNGEIVPLTDVLLARIRRITDDLNQQGLRVVAVAHKVMPSRTQGYGVTDESSLILA
GYIAFLDPPKESTAPALDALQRKGVTVKILTGDNPLVARKVCKDVGLQADNVVIGSDIDA
LDDVQLLNVARETTVFAKLTPMHKERIVRVLRGEGHVVGFMGDGINDAPALRAADIGISV
DSAVDIAKEAADIILLEKSLMVLEQGVTEGRRTFANMLKYIKMTASSNFGNVFSVLVASA
FLPFLPMLPLHLLIQNLIYDVSQVAIPFDNVDEEQLAKPQRWNAGDIGRFMVFFGPISSV
FDILTFSLMWWVFKANTVEAQTLFQSGWFIEGLLSQTLIVHMIRTRKVPFIQSRASWPLC
IMTLMVVITGISLIYSPVAGFLQLQALPLSYFPWLIAILAGYMVLTQCVKGWFVRRYGWQ