Protein Info for EX31_RS08335 in Rahnella sp. WP5

Annotation: cyclic di-GMP phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 PF00989: PAS" amino acids 113 to 178 (66 residues), 24.2 bits, see alignment E=4.2e-09 TIGR00229: PAS domain S-box protein" amino acids 114 to 232 (119 residues), 48 bits, see alignment E=1.4e-16 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 233 to 392 (160 residues), 111.8 bits, see alignment E=2.7e-36 PF00990: GGDEF" amino acids 236 to 389 (154 residues), 146.2 bits, see alignment E=1.2e-46 PF00563: EAL" amino acids 412 to 644 (233 residues), 232.6 bits, see alignment E=6.9e-73

Best Hits

Swiss-Prot: 60% identical to PDER_ECOLI: Cyclic di-GMP phosphodiesterase PdeR (pdeR) from Escherichia coli (strain K12)

KEGG orthology group: K14051, cyclic di-GMP phosphodiesterase Gmr [EC: 3.1.4.52] (inferred from 100% identity to rah:Rahaq_0530)

MetaCyc: 60% identical to cyclic di-GMP phosphodiesterase PdeR (Escherichia coli K-12 substr. MG1655)
Cyclic-guanylate-specific phosphodiesterase. [EC: 3.1.4.52]

Predicted SEED Role

No annotation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (672 amino acids)

>EX31_RS08335 cyclic di-GMP phosphodiesterase (Rahnella sp. WP5)
MVDSQDASLLYLHFGTPRPYWRLGSDSNALELSAEKGVVNIAISLTQWQAGKIRQLTGIT
SSFTLDITLFGSPARLYLVGRKIDSKAWAGTASAYQDTESVAHDLEVGLTFAEQVVSEAN
SVIVIIDQEGKIHRFNRLSEEYTGLREEDVIGRSAYDLFMSMEEGLASRQNINGFFQHQQ
SYEVERWINTVKGQRLFLFRNKFVHSGSGKQQKYLICSGSDITDERKAQERLRVLANTDL
VTGFANRNALQESLARALIERGENNVGLIYLDLDNFKKVNDAYGHMFGDHLLRAVSDAIQ
DCLSPNDKLARLGGDEFIILSTHAEIKALEALSQRVLRRLSLPFRIGLIEVYTGCSAGIA
ICPEHGTDGDILLQNADTAMYAAKEKGKNVHCVFSPEMNEKASEYMWLDTHLRKALSEKD
QLVLHYQPKVSLYGTVQSVEALLRWNSPERGLIQPQSFIPYAEESGLITPVGKWVIETAT
RQATEWQKKGLNLRVAVNLSGRQLNNSAFITTLSDAMKNAGMKGCLLDFELTESCLVEDE
GAAITMINQLHKLGAQVHLDDFGTGYSSLSQLARIPLDCIKLDQSFIRGINDNPVSQALV
RAIVAVAKTLQLTVVAEGVETAEEEKLIDEIGVDSKQGFLYAKPMPANELEHWLEEKMIA
SPNVFKHKAKQK