Protein Info for EX31_RS08330 in Rahnella sp. WP5

Annotation: beta-glucosidase BglX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 765 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 47 to 350 (304 residues), 269.3 bits, see alignment E=8.4e-84 PF01915: Glyco_hydro_3_C" amino acids 395 to 648 (254 residues), 206.8 bits, see alignment E=5.2e-65 PF14310: Fn3-like" amino acids 686 to 754 (69 residues), 80.6 bits, see alignment E=1.1e-26

Best Hits

Swiss-Prot: 69% identical to BGLX_SALTY: Periplasmic beta-glucosidase (bglX) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 100% identity to rah:Rahaq_0531)

MetaCyc: 68% identical to beta-D-glucoside glucohydrolase, periplasmic (Escherichia coli K-12 substr. MG1655)
Beta-glucosidase. [EC: 3.2.1.21]

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (765 amino acids)

>EX31_RS08330 beta-glucosidase BglX (Rahnella sp. WP5)
MNTHHMLGALVLLVSSASSLAVETTGIPQDQVKERNAFIADLMKKMTLDEKIGQLNLVTV
GPEYQKEAIMADIRAGKVGGVFNTVTKPDIRRMQDQVQYSRLKIPPFYAYDVVHGQRTIF
PISLGLAASWDMQAVATSARISAQETAADGLNMTFSPMVDITRDPRWGRVSEGFGEDTYL
TSRLAHEMVTGYQGKDPSAPDAVMANVKHYALYGAVEGGREYNTVDMSLTRMFNDYMPPY
KAAVDAGAGGVMVALNSVNGIPATSNTWLLKDILRDDWQFKGLTVSDHGAIGGLVKHGVA
EDDRQAAAMALKAGVDMDMADNMYGKYLKGLLADGMVSQKDIDRAVRDVLAAKWDMGLFV
DEYRHLGPQSSDPADTNAESRLHRQEAREVARTSLVLLKNNNQTLPLNKKGTIAVVGALA
DSKRDVMGSWSAAGKAAQAVTVLQGMKDVLGDKGTILYARGANVTNDQALVDFLNSYEKQ
VINDPRKPQDMIDEAVKTAEKADVVVAVVGEAQGMAHEASSRSEINIPQSQRDLLKALKA
TGKPLVVVLMNGRPLTLSWENEISDAMLETWFSGTEGGHAIADVLFGDYNPSGKLPMTFP
RSVGQIPLYNSVLNTGRPFNPQHPDKYTTRYFDITNGPLFPFGYGLSYTSFSMSDVSLSS
TTMDAGHPLTASVTVKNTGKVSGATIVQMYLQDVTASISRPVKELKNFEKINLQPGEEKV
VTFAITEKDLRFYNDKLKWASEPGKFNVFVGLDSQDVKQTSFLLK