Protein Info for EX31_RS08300 in Rahnella sp. WP5

Annotation: maltose ABC transporter permease MalF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 514 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 38 to 56 (19 residues), see Phobius details amino acids 67 to 89 (23 residues), see Phobius details amino acids 280 to 306 (27 residues), see Phobius details amino acids 317 to 339 (23 residues), see Phobius details amino acids 371 to 392 (22 residues), see Phobius details amino acids 421 to 443 (23 residues), see Phobius details amino acids 485 to 506 (22 residues), see Phobius details PF20872: MalF_N_TM" amino acids 12 to 59 (48 residues), 78.5 bits, see alignment 3.6e-26 PF14785: MalF_P2" amino acids 98 to 260 (163 residues), 196.3 bits, see alignment E=5.1e-62 PF00528: BPD_transp_1" amino acids 298 to 501 (204 residues), 70.6 bits, see alignment E=2.2e-23

Best Hits

Swiss-Prot: 76% identical to MALF_YERPE: Maltose/maltodextrin transport system permease protein MalF (malF) from Yersinia pestis

KEGG orthology group: K10109, maltose/maltodextrin transport system permease protein (inferred from 100% identity to rah:Rahaq_0538)

MetaCyc: 75% identical to maltose ABC transporter membrane subunit MalF (Escherichia coli K-12 substr. MG1655)
ABC-16-RXN [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]

Predicted SEED Role

"Maltose/maltodextrin ABC transporter, permease protein MalF" in subsystem Maltose and Maltodextrin Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (514 amino acids)

>EX31_RS08300 maltose ABC transporter permease MalF (Rahnella sp. WP5)
MPVAKKRPFSNALKWLIIALLACFTGYLIVLMYAQGEYLFAVLTLILSGVGLYIYGNRRA
YSWRYVYPGLAGMSLFVLFPLICTIAIAFTNYSSTNQLTFERAQSVLMGREFQGGKVLNF
ALFPAGDRWQLALTSADNSETLVSPPFAFNASAPQTLDMLPQTAPSAEKAGLRVVTQNRL
ALGQISAKLPDGETLRMSSLRQFSGTQPLYKLGEDKKQLTNVQTGTIYRPNMDTGFYQAV
NAQGEWQAEKLSPGFTVGIGWGNFLRVLHDEGIQKPFLSIFVWTVVFALLSVVLTVAVGM
VLACVVQWEELKGKAAYRILLILPYAVPAFISILIFKGLFNQSFGEINLMLSALFGIKPA
WFSDPWTAKSMIIIVNTWLGYPYMMILCMGLLKAIPDDLYEASAMDGATPMQNFFRITLP
LLIKPLTPLMIASFAFNFNNFVLIQLLTNGGPDMLGTTTPAGHTDLLVSYTYRIAFEGGG
GQDFGLAAAIATLIFLLVGALAVVNLKASRMKFD