Protein Info for EX31_RS08295 in Rahnella sp. WP5

Annotation: maltose/maltodextrin ABC transporter substrate-binding protein MalE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF01547: SBP_bac_1" amino acids 49 to 311 (263 residues), 146.1 bits, see alignment E=2.5e-46 PF13416: SBP_bac_8" amino acids 53 to 347 (295 residues), 129.8 bits, see alignment E=1.8e-41

Best Hits

Swiss-Prot: 83% identical to MALE_ECOLI: Maltose/maltodextrin-binding periplasmic protein (malE) from Escherichia coli (strain K12)

KEGG orthology group: K10108, maltose/maltodextrin transport system substrate-binding protein (inferred from 100% identity to rah:Rahaq_0539)

MetaCyc: 83% identical to maltose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-16-RXN [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]; 7.5.2.1 [EC: 7.5.2.1]

Predicted SEED Role

"Maltose/maltodextrin ABC transporter, substrate binding periplasmic protein MalE" in subsystem Bacterial Chemotaxis or Maltose and Maltodextrin Utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (399 amino acids)

>EX31_RS08295 maltose/maltodextrin ABC transporter substrate-binding protein MalE (Rahnella sp. WP5)
MTLSIKKIVKHTLALSALATLVLSSSAFAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDT
GIKVTVEHPDKLEEKYPQVAATGGGPDIIFWAHDRFGGYAQSGLLAEISPDQTFKDKLFP
FTWDAVTFNGKLIGYPVAVEALSLIYNKDIIKEAPKTWEEIPALDKELRATGKSAIMWNL
QEPYFTWPIIAADGGYAFKYENGKYNIKDTGVNNAGSQAGLQFIIDLVKNKHINADTDYS
IAEAAFNKGQTAMTIDGPWAWSNLDKSKINYGVTLLPTFHGKPSKPFVGVLTAGINAASP
NKELAKEFLENYLITDDGLAVVNKDKPLGAVALKSYQEKLEKDPRIAATMANSKNGEIMP
NIPQMSAFWYAERSAIINAVNGRQTVPQALKDVETRITK