Protein Info for EX31_RS08060 in Rahnella sp. WP5

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 757 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 10 to 743 (734 residues), 1318 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 33 to 469 (437 residues), 458 bits, see alignment E=3.4e-141 PF03989: DNA_gyraseA_C" amino acids 597 to 633 (37 residues), 20.6 bits, see alignment 2.4e-08 amino acids 645 to 695 (51 residues), 20.3 bits, see alignment 3e-08

Best Hits

Swiss-Prot: 82% identical to PARC_SALTY: DNA topoisomerase 4 subunit A (parC) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 85% identity to ddd:Dda3937_01254)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (757 amino acids)

>EX31_RS08060 DNA topoisomerase IV subunit A (Rahnella sp. WP5)
MSEMTHDGTERLALHTFTENAYLNYSMYVIMDRALPFIGDGLKPVQRRIIYAMSELGLSN
NAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDGQGNWGAPDDPKSFA
AMRYTESRLSKYAEVLLRELGQGTVDYVPNFDGTMQEPKMLPARLPNILLNGTTGIAVGM
ATDIPPHNIREVAAAAVALLEKPNSSLEDLLEFVQGPDFPTEAEIITPRNEIRKMYESGK
GSVRMRAVWHKEDGSAVITALPHQVSGAKVLEQIASQMRAKKLPMVEDLRDESDHENPTR
LVVVPRSNRIDLDQVMNHLFATTDLERSYRINMNMIGLDHRPQVKGLLEILTEWLAYRRD
TVRRRLNFRLDKVLKRLHILEGLLTAFLNIDEVIHIIRTEDEPKPVLMQRFELSDTQAEA
ILELKLRHLAKLEETKIRGEQDELAKERDQLQALLASERKLSTLIRKEIQADAEAFGDER
RSPITEREEARAMSEHDFIPSEPVTIVLSESGWVRSAKGHDIDASGLSYKAGDSFRAAAK
GKSNQPVVFMDSTGRSYALDPTTLPSARGQGEPLTGKLTPPPGATIEHVLMAADDQKLLM
ASDAGYGFVCTFNDLVARNRAGKAMITLPDNAKAFTPMELHGSDDMLLSITAAGRMLLFP
VADLPQLSKGKGNKIVSIPAAQAASGEDKLAWLLVLPPQTSITLHVGKRKLVLRPEDLQK
FRAERGRKGTLLPRGLQRIDRVEVDSPVRISGGDSEE