Protein Info for EX31_RS08010 in Rahnella sp. WP5

Annotation: TRAP transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF03480: DctP" amino acids 31 to 304 (274 residues), 248 bits, see alignment E=6.1e-78 TIGR00787: TRAP transporter solute receptor, DctP family" amino acids 35 to 286 (252 residues), 215.9 bits, see alignment E=3.2e-68

Best Hits

Swiss-Prot: 38% identical to DCTP1_POLSJ: Solute-binding protein Bpro_3107 (Bpro_3107) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: None (inferred from 100% identity to rah:Rahaq_0649)

Predicted SEED Role

"TRAP-type C4-dicarboxylate transport system, periplasmic component"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (325 amino acids)

>EX31_RS08010 TRAP transporter substrate-binding protein (Rahnella sp. WP5)
MKMQKALLSLCLSSATLLFTHGVSAQTLKAADVHPQGYPNVVAVQHMGEKLQKQTDGRLE
IKTFPGGVLGDEKQEIEQAQLGALDIIRVSMTPVASILPEINVFTLPYIFRDEDHMHKVL
DGQIGKEIGDKITNNPNSRLVFLGWMDGGTRNLITKKPVVKPEDLHGMKIRVQGSPIALA
TLKAMGANPVAMGVSEVFSGMQTGVIDGTENNPPTFVAHNYLPVVKNYTYSRHFIIPELF
LYSKVKWDKLKPEDQQLILKLAKEAQDEQRELWKAYNEKALATMKAGGVKFYEIDPATYV
KATQSVRDEFGKDHQDLMQQIADVK