Protein Info for EX31_RS07955 in Rahnella sp. WP5

Annotation: GTP diphosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 745 PF13328: HD_4" amino acids 46 to 191 (146 residues), 154.5 bits, see alignment E=6.6e-49 TIGR00691: RelA/SpoT family protein" amino acids 64 to 741 (678 residues), 631.4 bits, see alignment E=9.5e-194 PF04607: RelA_SpoT" amino acids 251 to 360 (110 residues), 143.8 bits, see alignment E=8.8e-46 PF02824: TGS" amino acids 407 to 466 (60 residues), 75.9 bits, see alignment 7e-25 PF19296: RelA_AH_RIS" amino acids 484 to 646 (163 residues), 52.7 bits, see alignment E=2e-17 PF13291: ACT_4" amino acids 665 to 741 (77 residues), 64.2 bits, see alignment E=5.2e-21 PF01842: ACT" amino acids 669 to 734 (66 residues), 40.3 bits, see alignment E=7.6e-14 PF22629: ACT_AHAS_ss" amino acids 672 to 738 (67 residues), 29.3 bits, see alignment E=2.7e-10

Best Hits

Swiss-Prot: 84% identical to RELA_ECO57: GTP pyrophosphokinase (relA) from Escherichia coli O157:H7

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 87% identity to eca:ECA3569)

MetaCyc: 84% identical to GDP/GTP pyrophosphokinase (Escherichia coli K-12 substr. MG1655)
GTP diphosphokinase. [EC: 2.7.6.5]; 2.7.6.5 [EC: 2.7.6.5]

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (745 amino acids)

>EX31_RS07955 GTP diphosphokinase (Rahnella sp. WP5)
MVAVRSAHLNTAGEFALDDWINSLGLSSQQSCERLAETWRYCEQQTQGHPDASLLLWRGL
EMVEILSTLSMDNDSMRAALLFPLVDDGIIDEDTLTQQFGKAITDLVHGVRDMDAIRQLK
ATQNDSMASEQVDNVRRMLLAMVEDFRCVVLKLAERIAHLREVKDAPEEERVLAAKECTN
IYAPLANRLGIGQLKWELEDFCFRYLHPDEYKRIAKLLHERRIDREQYIDDFVSTVKNAM
AEEGIKVDVYGRPKHIYSIWRKMQKKSLSFDELFDVRAVRIVVERLQDCYAALGIVHTHF
RHLPDEFDDYVANPKPNGYQSIHTVVLGPRGKTLEVQIRTRQMHEDAELGVAAHWKYKEG
TAVVGGRSGGYEGRIAWLRKLIAWQEEMADSGEMLDEVRSQVFDDRVYVFTPKGDVIDLP
MGSTPLDFAYHIHSDVGHRCIGAKIGGRIVPFTYQLHMGDQIEIITQKHANPSRDWLNPS
LGYVTTSRGRSKIQNWFRKQDRDKNIIAGKQILDDELDRLDISIKDAEKLLLPRYNVNSL
DEVLAAIGNGDIRLNQMVNFLQSQLKKPSAEELDKEALRQLTQKTTPPPTRTKDNGRVVV
EGVGNLMHHIARCCQPIPGDEIVGYITQGRGISIHRADCDQLEDLRSNAPERVVDAVWGE
SYSSGYSLVVRVVANDRSGLLRDITTILANEKVNVLGVASRSDTRQQLATIDMDIEIYNP
QVLGRVIAKLNQLPDVIEAKRLHGS