Protein Info for EX31_RS07900 in Rahnella sp. WP5

Annotation: assimilatory sulfite reductase (NADPH) hemoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02041: sulfite reductase (NADPH) hemoprotein, beta-component
  • amino acids 26 to 566 (541 residues), 955.5 bits, E=3.6e-292
• PF03460: NIR_SIR_ferr
  • amino acids 87 to 146 (60 residues), 49.5 bits, 2.9e-17
  • amino acids 361 to 419 (59 residues), 41.8 bits, 7.4e-15
• PF01077: NIR_SIR
  • amino acids 180 to 337 (158 residues), 142.4 bits, E=8.4e-46

Best Hits

Swiss-Prot: 87% identical to CYSI_SERP5: Sulfite reductase [NADPH] hemoprotein beta-component (cysI) from Serratia proteamaculans (strain 568)

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 100% identity to rah:Rahaq_0672)

MetaCyc: 81% identical to sulfite reductase, hemoprotein subunit (Escherichia coli K-12 substr. MG1655)
Sulfite reductase (NADPH). [EC: 1.8.1.2]

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (578 amino acids)

>EX31_RS07900 assimilatory sulfite reductase (NADPH) hemoprotein subunit (Rahnella sp. WP5)
MSNKLWSELNPGPGPLIVDAPLADAERLKKESNFLRGTITEDLKDGLTGGFNGDNFLLIR
FHGMYQQDDRDIRAERAEQKLEPRHAMMLRCRLPGGVMTPAQWLGIDKFAEDSTLYGSIR
ITNRQTFQFHGILKPNVKPVHQLLNKLGLDALATANDVNRNVLCTSNPVESELHQEAYQW
AKKISEHLLPRTRAYAEVWLDQEKVETTDEEPILGATYLPRKFKTTVVIPPQNDVDLHAN
DLNFVAIADNGKLVGFNVLVGGGLSIAHGDKATYARTASELGFIPLENTLAVAEAVVTTQ
RDWGNRTNRKNAKTKYTLERVGVPVFKAEVEKRAGIQFGDIRPYEFTGRGDRIGWVKGID
KQWHLTLFIENGRILDYPGKPLKTGLAEIAKIHKGDFRLTANQNLIVAGVAEKDKDAIET
LARNHGLIDDGVTEQRKNSMACVSYPTCPLAMAEAERFLPEFVTKVEGIMHGHGVGDEHI
VLRVTGCPNGCGRALLAEIGLVGKAMNRYNLHLGGNRAGTRIPRMYRENISDAEILGIID
ELVGRWSKERENGEGFGDFVIRAGIIKPVLDPARDFYD