Protein Info for EX31_RS07840 in Rahnella sp. WP5

Annotation: 5'/3'-nucleotidase SurE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00087: 5'/3'-nucleotidase SurE
  • amino acids 2 to 233 (232 residues), 251.9 bits, E=3.2e-79
• PF01975: SurE
  • amino acids 4 to 182 (179 residues), 204.5 bits, E=7.1e-65

Best Hits

Swiss-Prot: 85% identical to SURE_SERP5: 5'/3'-nucleotidase SurE (surE) from Serratia proteamaculans (strain 568)

KEGG orthology group: K03787, 5'-nucleotidase [EC: 3.1.3.5] (inferred from 100% identity to rah:Rahaq_0684)

MetaCyc: 80% identical to broad specificity 5'(3')-nucleotidase and polyphosphatase (Escherichia coli K-12 substr. MG1655)
RXN-14142 [EC: 3.1.3.89]; 3'-nucleotidase. [EC: 3.1.3.89, 3.1.3.6]; 3.1.3.6 [EC: 3.1.3.89, 3.1.3.6]; 3.1.3.6 [EC: 3.1.3.89, 3.1.3.6]; 5'-nucleotidase. [EC: 3.1.3.89, 3.1.3.6, 3.1.3.5]

Predicted SEED Role

"5-nucleotidase SurE (EC 3.1.3.5)" in subsystem Folate Biosynthesis (EC 3.1.3.5)

MetaCyc Pathways

• purine nucleotides degradation II (aerobic) (11/11 steps found)
• superpathway of purines degradation in plants (15/18 steps found)
• ureide biosynthesis (7/7 steps found)
• adenosine nucleotides degradation II (5/5 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• guanosine nucleotides degradation III (4/4 steps found)
• inosine 5'-phosphate degradation (4/4 steps found)
• NAD salvage pathway III (to nicotinamide riboside) (3/3 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• NAD salvage (plants) (5/11 steps found)
• tunicamycin biosynthesis (2/9 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.5

Use Curated BLAST to search for 3.1.3.5 or 3.1.3.6 or 3.1.3.89

Protein Sequence (255 amino acids)

>EX31_RS07840 5'/3'-nucleotidase SurE (Rahnella sp. WP5)
MMRILLSNDDGVLAPGIQALASALREFADVTLVAPDRNRSGASNALTLDGPLRIQSYPNG
DTAVINGTPTDCVYLGVNTLMRPKPDIVVSGINAGPNLGDDVIYSGTVAAAMEGRHLGFP
ALAVSLDGHQHYDTAAIITCRILRALAREPLRTGKILNINVPDLPLSQIKGIKVTRCGSR
HPAEQVFCQQDPRGQDLYWIGPPGDKFDVAPDTDFAAVAQGYVSVTPLQVDLTAYAAQDV
VVSWLEKAGVSANGE