Protein Info for EX31_RS07315 in Rahnella sp. WP5

Annotation: peptide chain release factor 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 360 TIGR00019: peptide chain release factor 1" amino acids 1 to 358 (358 residues), 573.9 bits, see alignment E=5.9e-177 PF03462: PCRF" amino acids 12 to 203 (192 residues), 263 bits, see alignment E=1.8e-82 PF00472: RF-1" amino acids 216 to 321 (106 residues), 146.4 bits, see alignment E=3.3e-47

Best Hits

Swiss-Prot: 88% identical to RF1_SERP5: Peptide chain release factor 1 (prfA) from Serratia proteamaculans (strain 568)

KEGG orthology group: K02835, peptide chain release factor 1 (inferred from 100% identity to rah:Rahaq_2276)

Predicted SEED Role

"Peptide chain release factor 1" in subsystem LMPTP YwlE cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (360 amino acids)

>EX31_RS07315 peptide chain release factor 1 (Rahnella sp. WP5)
MKPSIVAKLEALQERHEEVQAMLGDAGVIADQDKFRSLSREYSQLTAVSECFLAWRQAQE
DLETAEMMLDDPEMREMAQDEIKECKANIEELEEKLQVLLLPKDPDDERGCFLEIRAGTG
GDEAAIFAGDLFRMYSRYAEMRRWRVEIVSASEGEHGGYKEVIAKISGEGAYGQFKFESG
GHRVQRVPETESQGRIHTSACTVAVMPEIPEAELPEINAGDLRIDTFRSSGAGGQHVNTT
DSAIRITHIPTGIVVECQDERSQHKNKAKAMSVLGARIRAAEVAKRQAEEASTRRNLLGS
GDRSDRNRTYNFPQGRVTDHRINLTIYRLDEVMEGKLDSLIQPIVQEYQADQLAALSEQD