Protein Info for EX31_RS07170 in Rahnella sp. WP5

Annotation: glycogen debranching protein GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 760 TIGR02100: glycogen debranching enzyme GlgX" amino acids 63 to 753 (691 residues), 1075.3 bits, see alignment E=0 PF02922: CBM_48" amino acids 67 to 154 (88 residues), 67.8 bits, see alignment E=1.3e-22 PF00128: Alpha-amylase" amino acids 249 to 340 (92 residues), 39.3 bits, see alignment E=9.4e-14 PF21331: Isoamylase_C" amino acids 650 to 729 (80 residues), 27.3 bits, see alignment E=6e-10

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to rah:Rahaq_2247)

MetaCyc: 52% identical to isoamylase (Sulfolobus acidocaldarius)
Isoamylase. [EC: 3.2.1.68]

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.68

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (760 amino acids)

>EX31_RS07170 glycogen debranching protein GlgX (Rahnella sp. WP5)
MAKARSSKISKPAVTTEAIRTLPAHLQVDIAVDEFGVRIIEPYSDGEIVRSFDSGHTTRV
REGLPVPLGATWDGLGVNFALFSANATKVELCLFNEDGEQYDVIELPEYTDEVWHGYLPD
ARPGLLYAYRVHGPYAPHDGHRFNPAKLLLDPYAKQIVGELKWDDALFAYDLQSPKKDLH
IDNSDSAPFLPKCRVIDPAFSWARSRKRHIPWEKTLIYETHVRGYTMRHPAVPEHLRGTF
SGMSTPHIVDYIRSLGVTSVELMPVHAFTDDRHLQDKGLHNFWGYNTLSFFAPHPQYMAT
HTVNEFKQMIATLHAAGIEVILDVVYNHTAEGNELGPTLSFKGIDNASYYRLMPDNKRYY
INDTGTGNTLNLSHPRVLQMVTDSLRYWATEMQVDGFRFDLATILGRESYGFDEGCGFLD
TCRQDPILSQCKLIAEPWDCGPGGYQVGGFPPGWMEWNDRFRDTVRKFWRGDEGQLAQFV
TRLSGSADLFNHRGRKPYASVNFITAHDGFTLNDWVSYASKHNEANGEDGKDGSDNNISA
NYGVEGPTDDPAINELRLRQMRNMLATLLLSQGTPMLLAGDEFGRSQNGNNNAYCQDTEI
AWVNWDIGDKGQSLLLFTYRLIALRKRFPILHRGRFLTGALHEKLGVKDVSWFQPQGPEM
TDAAWKDPHAKCIAMLLDGRAQPTGLVRQGSLSTVLLLFNASHEDVVFTLPSVAHGESWR
HIFDTFQPDDINEHNFEFGREYVCTSRTVVLFELISTAKH