Protein Info for EX31_RS07080 in Rahnella sp. WP5

Annotation: NCS2 family permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 transmembrane" amino acids 20 to 42 (23 residues), see Phobius details amino acids 49 to 75 (27 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 129 to 155 (27 residues), see Phobius details amino acids 168 to 185 (18 residues), see Phobius details amino acids 192 to 213 (22 residues), see Phobius details amino acids 234 to 254 (21 residues), see Phobius details amino acids 282 to 304 (23 residues), see Phobius details amino acids 316 to 335 (20 residues), see Phobius details amino acids 342 to 363 (22 residues), see Phobius details amino acids 383 to 401 (19 residues), see Phobius details amino acids 411 to 429 (19 residues), see Phobius details PF00860: Xan_ur_permease" amino acids 17 to 391 (375 residues), 197.2 bits, see alignment E=1.9e-62

Best Hits

Swiss-Prot: 51% identical to ADEP_ECOLI: Adenine permease AdeP (adeP) from Escherichia coli (strain K12)

KEGG orthology group: K06901, putative MFS transporter, AGZA family, xanthine/uracil permease (inferred from 100% identity to rah:Rahaq_2228)

MetaCyc: 51% identical to adenine:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-447

Predicted SEED Role

"Xanthine/uracil/thiamine/ascorbate permease family protein" in subsystem Purine Utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>EX31_RS07080 NCS2 family permease (Rahnella sp. WP5)
MLEKLFKLKAHRTNVRTEIVAGLTTFLAMAYILFVNPSILGATGMDKGSVFVATCLAAAI
GSALMGFIANYPIALAPGMGLNAFFTYTVVLHMGYTWQIALGAVFLSACIFFALSIFKIR
EWIIRSIPLPLRSAIAAGIGLFLALIALENAGIVIANPATLVGLGDLTKPGPLFAMLGFI
LIVVLEARKITGAVLIGVLAVTVLAIALGYSPFGGVMSMPPSIAPTFMQLDIKGAFNVSL
ISVVFAFLFVDVFDNSGTLIGVTKRAGLADEDGNIPKMGRALVADSAAALFGSLLGTSTT
TSYIESAAGVSAGGRTGLTAIVVAILFLLSLFFAPLAGSVPAFATAPALLFIAVLMTSGL
AEIDWKDITVAAPVTVTALTMPFTYSIANGIAFGFITWTLAKLLTGRWRELNSALIVLSI
LFVIKLGWLSA