Protein Info for EX31_RS06760 in Rahnella sp. WP5

Annotation: urocanate hydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 562 TIGR01228: urocanate hydratase" amino acids 12 to 559 (548 residues), 958.5 bits, see alignment E=5.1e-293 PF17391: Urocanase_N" amino acids 13 to 139 (127 residues), 202 bits, see alignment E=3.9e-64 PF01175: Urocanase" amino acids 142 to 354 (213 residues), 292.4 bits, see alignment E=3e-91 PF17392: Urocanase_C" amino acids 357 to 551 (195 residues), 314.5 bits, see alignment E=3.4e-98

Best Hits

Swiss-Prot: 86% identical to HUTU_TRIRP: Urocanate hydratase from Trifolium repens

KEGG orthology group: K01712, urocanate hydratase [EC: 4.2.1.49] (inferred from 100% identity to rah:Rahaq_2164)

MetaCyc: 74% identical to urocanase subunit (Pseudomonas putida)
Urocanate hydratase. [EC: 4.2.1.49]

Predicted SEED Role

"Urocanate hydratase (EC 4.2.1.49)" in subsystem Histidine Degradation (EC 4.2.1.49)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.49

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (562 amino acids)

>EX31_RS06760 urocanate hydratase (Rahnella sp. WP5)
MNAPQKTAIARVVRAPQGTELSCQNWLIEAAYRMIQNNLDPDVAERPEDLVVYGGIGKAA
RNWPAFEGILDSLRKLREDETLLVQSGKPVGVFRTHTDAPRVLIANSNIVPHWANWDHFH
ELDKAGLMMYGQMTAGSWIYIGAQGIVQGTYETFAEAGRQHYNSDLRGKWILTAGLGGMG
GAQPLAGVLAGACVLAIECQESRIDFRLRTRYLDYKAHSIDEALAMIEKACAEKKAISVG
LMGNAAEVMPQLVARAKEGGLRPDIVTDQTSAHDPLNGYLPEGWSLEKWQDARLSDPQSV
VKAARASMAKHVQAMLDFHAMGIPTVDYGNNIRQVAKDEGVENAFDFPGFVPAYIRPLFC
EGKGPFRWVALSGDPEDIYKTDAKLKELFPDNANLINWLDMARERIAFQGLPARICWLGL
GERHIAGLAFNEMVRNGELKAPVVIGRDHLDTGSVASPNRETEAMKDGSDAVSDWPLLNA
LLNTAGGATWVSLHHGGGVGMGFSQHAGMVIVADGTREADARLDRVLWNDPATGVMRHAD
AGYEQAKDCAERHHLNLPMLAK