Protein Info for EX31_RS06650 in Rahnella sp. WP5

Annotation: L-arabinose ABC transporter ATP-binding protein AraG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 528 PF00005: ABC_tran" amino acids 42 to 191 (150 residues), 125.5 bits, see alignment E=2.4e-40 amino acids 293 to 446 (154 residues), 74.9 bits, see alignment E=1.1e-24

Best Hits

Swiss-Prot: 84% identical to ARAG_PECAS: Arabinose import ATP-binding protein AraG (araG) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K10539, L-arabinose transport system ATP-binding protein [EC: 3.6.3.17] (inferred from 100% identity to rah:Rahaq_2142)

MetaCyc: 81% identical to arabinose ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.17

Use Curated BLAST to search for 3.6.3.17 or 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (528 amino acids)

>EX31_RS06650 L-arabinose ABC transporter ATP-binding protein AraG (Rahnella sp. WP5)
MTQQSFDNKTPLLTPSLTEFDAARPYLEFHDIGKTFPGVKALDGISFNCYAGQIHALMGE
NGAGKSTLLKILSGNYQPSQGNIHIKGQPVAFSHTTDALDAGVAIIYQELHLVPEMTVAE
NIYLGQLPSKHGFVNRKLLRYEAKLQLEHLGLDIDPDTPLKYLSIGQWQMVEIAKALARN
AKIIAFDEPTSSLSAREIEQLFRVIRELRAEGRVILYVSHRMEEIFALSDAVTVFKDGRY
VRTFDDMTQVDNSKLVQAMVGRNLDDIYGYAPRPHGEIRLELDEVKAPGVRTPISLTVRQ
GEIVGLFGLVGAGRSELMKGLFGGTRITGGTVRLDGVALNIRKPADAIRQGIMLCPEDRK
ADGIIPVHSVRDNINISARRKTVKAGCLINNAWEAKNARKHIDALNIKTPSDQQLIMNLS
GGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYSLAKQGIAVLFASSDLPE
VLGLADRVVVMREGAISGELLHDDATEEKALGLAMLRTPVLDSAHDAA