Protein Info for EX31_RS06185 in Rahnella sp. WP5

Annotation: ribose ABC transporter substrate-binding protein RbsB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF00532: Peripla_BP_1" amino acids 27 to 288 (262 residues), 83.6 bits, see alignment E=3.5e-27 PF13407: Peripla_BP_4" amino acids 29 to 281 (253 residues), 197.8 bits, see alignment E=4.9e-62 PF13458: Peripla_BP_6" amino acids 58 to 251 (194 residues), 38 bits, see alignment E=3.3e-13 PF13377: Peripla_BP_3" amino acids 159 to 284 (126 residues), 48.6 bits, see alignment E=2e-16

Best Hits

Swiss-Prot: 82% identical to RBSB_ECOLI: Ribose import binding protein RbsB (rbsB) from Escherichia coli (strain K12)

KEGG orthology group: K10439, ribose transport system substrate-binding protein (inferred from 100% identity to rah:Rahaq_4398)

MetaCyc: 82% identical to ribose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-28-RXN

Predicted SEED Role

"Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1)" in subsystem D-ribose utilization (TC 3.A.1.2.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>EX31_RS06185 ribose ABC transporter substrate-binding protein RbsB (Rahnella sp. WP5)
MNMKKLAVWVSAAAITASFSANVLAKDTIALVVSTLNNPFFVSMKDGAQKEADKLGYNLV
VLDSQNNPAKELANVQDLTVRGTKLMLINPTDSDAVGNAVAMANQAKIPVITLDRQATKG
VVASHVASDNAFGGKMAGDYIAKKLGENAKVIELEGIAGTSVARERGKGFAQAAEKHHFQ
ILASQPADFDRTKGLNVMQNLLTAHQDVQAVFAQNDEMALGALRALQTAGKTDVLVVGFD
GTDDGIKAVNSGKMGATVAQRPEQIGVIGVQTADKVLKGEKVPAVIPVDLKLVAQP