Protein Info for EX31_RS06085 in Rahnella sp. WP5

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 7 to 455 (449 residues), 666 bits, E=1.4e-204
• PF12804: NTP_transf_3
  • amino acids 8 to 120 (113 residues), 74 bits, E=3.2e-24
• PF00483: NTP_transferase
  • amino acids 8 to 211 (204 residues), 44.8 bits, E=2.4e-15
• PF00132: Hexapep
  • amino acids 269 to 300 (32 residues), 29 bits, see alignment (E = 1.2e-10)
  • amino acids 395 to 429 (35 residues), 37 bits, 3.6e-13
• PF14602: Hexapep_2
  • amino acids 395 to 429 (35 residues), 33.7 bits, 4.9e-12

Best Hits

Swiss-Prot: 82% identical to GLMU_ENT38: Bifunctional protein GlmU (glmU) from Enterobacter sp. (strain 638)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to rah:Rahaq_4418)

MetaCyc: 80% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• peptidoglycan recycling I (14/14 steps found)
• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (7/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (456 amino acids)

>EX31_RS06085 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Rahnella sp. WP5)
MSNSALSVVILAAGKGTRMYSNLPKVLHPLAGKPMVQHVIDAATQVGARNVHLVYGHGGD
LLKKNLAGSDLNWVLQAEQLGTGHAMQQAAPFFADDEDVLMLYGDVPLISVDTLNELLKA
KPDGGIGLLTVVLDDPTGYGRIVRENGIVTGIIEQKDASEEQLKIQEINTGILVANGASF
KRWLGQLNNNNAQGEYYITDIIAMAHAEGHLIATVHPARNSEVDGVNNRLQLSRLERVYQ
SEQAERLLLEGVMLMDPARFDLRGELSHGIDVLIDTNVIIEGHVKLGDRVKIGAGCVLKN
CVIGDDCEVSPYSVFEDAVLESGCTVGPFARLRPGAELAEGAHVGNFVEIKKARLGKGSK
AGHLSYLGDADIGDNVNIGAGTITCNYDGVNKHKTIIGNDVFVGSDSQLVAPVTVGNNVT
IAAGTTVTRDVPDNGLLLSRVPQVHKPDWQKPVKKK