Protein Info for EX31_RS05965 in Rahnella sp. WP5

Annotation: sugar-phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 TIGR01484: HAD hydrolase, family IIB" amino acids 5 to 237 (233 residues), 110.4 bits, see alignment E=1.4e-35 PF05116: S6PP" amino acids 5 to 81 (77 residues), 23.3 bits, see alignment E=9e-09 amino acids 168 to 246 (79 residues), 39.9 bits, see alignment E=7.5e-14 TIGR00099: Cof-like hydrolase" amino acids 5 to 263 (259 residues), 225.4 bits, see alignment E=9.1e-71 PF08282: Hydrolase_3" amino acids 6 to 263 (258 residues), 222.5 bits, see alignment E=1.6e-69

Best Hits

Swiss-Prot: 65% identical to YIDA_SHIFL: Sugar phosphatase YidA (yidA) from Shigella flexneri

KEGG orthology group: K07024, (no description) (inferred from 100% identity to rah:Rahaq_0005)

MetaCyc: 65% identical to sugar phosphatase YidA (Escherichia coli K-12 substr. MG1655)
Sugar-phosphatase. [EC: 3.1.3.23]

Predicted SEED Role

"Promiscuous sugar phosphatase YidA, haloacid dehalogenase-like phosphatase family"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (271 amino acids)

>EX31_RS05965 sugar-phosphatase (Rahnella sp. WP5)
MAIELIAIDMDGTLLNPQHEVTPAVKKALSEARAKGVQIVLATGRPFVGVQRYLMELDLQ
QDGCYCITNNGALVHNAKDGSVIAEVALKIEDYHYFEQLARDLGVHFHALTKTDLFTANR
HISKYSVHESFTTGIPLQFCPAEEMDPALTFPKVMMIDDPDLLDRAISQLPAEAKERYTI
MKSSPYFLEILDKRVNKGEGVKVLAEKLGIARESVMTLGDQENDIAMLEYAGIGVAMGNA
LDSVKAVSQFVTKTNTEDGVAFAVEKFVLNV